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Development of species-specific ISSR-derived SCAR marker for early discrimination between Cinnamomum verum and Cinnamomum cassia

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Abstract

Background

Cinnamomum verum (true cinnamon) and Cinnamomum cassia (cassia cinnamon) are two important species belonging to family Lauraceae. These species are recognized by morphological, chemical composition and essential oil contents. The appropriate identification of species would be considerably improved by a genetic method. The main objective of the present study was to develop molecular markers distinguishing between C. verum and C. cassia.

Methods and results

A total 71 ISSR (Inter simple sequence repeat) and four universal barcoding (ITS, rbcL, matK, and psbA-trnH) genes were used to distinguish both the species. No sequence variation was observed between the two species for any DNA barcode gene. However, one ISSR i.e. ISSR-37 showed a clear distinction between the species and produced 570 bp and 746 bp amplicons in C. verum and C. cassia, respectively. The polymorphic bands were converted into species-specific SCAR markers. The SCAR-CV was specific to C. verum and amplified 190 bp band, however there was no amplification seen in the C. cassia samples.

Conclusion

The SCAR marker generated in this study can be employed as efficient, economical, and reliable molecular tool for the identification of C. verum.

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Data Availability

All data needed to support the conclusions are included in this article. Additional data related to this paper can be requested from the corresponding author.

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Acknowledgements

This study was supported by the Council of Scientific and Industrial Research (CSIR) for providing funds (MLP-201, MLP-202), HG thanks CSIR for Junior Research Fellowship. Thanks are also to Dr. Balasaheb Sawant Konkan Krishi Vidyapeeth, Dapoli, Maharashtra and ICAR-IISR, Kozhikode, Kerala for providing plant material of C. cassia and C. verum, respectively. This manuscript represents CSIR-IHBT communication number 5309.

Funding

This study was funded by the Council of Scientific and Industrial Research (CSIR) for providing funds (MLP-201, MLP-202).

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Authors and Affiliations

  1. Biotechnology, Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, 176061, India

    Himanshi Gangwar, Vijay Gahlaut & Vandana Jaiswal

  2. Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India

    Himanshi Gangwar, Ramesh Chauhan, Satbeer Singh & Vandana Jaiswal

  3. Department of Biotechnology and University Center for Research and Development, Chandigarh University, Mohali, Punjab, 140413, India

    Vijay Gahlaut

  4. Agrotechnology, Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, 176061, India

    Ramesh Chauhan & Satbeer Singh

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  1. Himanshi Gangwar
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  2. Vijay Gahlaut
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  3. Ramesh Chauhan
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  4. Satbeer Singh
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Contributions

HG- data acquisition, wet-lab experiments, analysis, writing; VG- data acquisition, analysis; RC-plant material and sampling; SS-Conceptualization, Plant material and sampling, writing and editing, VJ-Conceptualization, Supervision, Data analysis and result interpretation, writing and editing.

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Correspondence to Vandana Jaiswal.

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Gangwar, H., Gahlaut, V., Chauhan, R. et al. Development of species-specific ISSR-derived SCAR marker for early discrimination between Cinnamomum verum and Cinnamomum cassia. Mol Biol Rep 50, 6311–6321 (2023). https://doi.org/10.1007/s11033-023-08578-z

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