Abstract
Background
Prostate cancer is the second most prevalent and the fifth deadliest cancer among men worldwide. To improve radiotherapy outcome, we investigated the effects of 7-geranyloxycoumarin, also known as auraptene (AUR), on radiation response of prostate cancer cells.
Methods and results
PC3 cells were pretreated with 20 and 40 µM AUR for 24, 48 and 72 h, followed by X-ray exposure (2, 4 and 6 Gy). After 72 h recovery, cell viability was determined by alamar Blue assay. Flow cytometric analysis was performed to assess apoptosis induction, clonogenic assay was carried out to investigate clonogenic survival, and the expression of P53, BAX, BCL2, CCND1 and GATA6 was analyzed by quantitative polymerase chain reaction (qPCR). Cell viability assay indicated that toxic effects of radiation was enhanced by AUR, which was also confirmed by increased numbers of apoptotic cells and reduced amount of survival fraction. The qPCR results demonstrated significant induction of P53 and BAX, while the expression of BCL2, GATA6, and CCND1 was significantly downregulated.
Conclusion
The findings of the present study indicated, for the first time, that AUR improved radio sensitivity in prostate cancer cells, and thus, has the potential to be used in future clinical trials.
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Data Availability
All data generated or analyzed during this study are included in this article.
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Funding
This work was supported by the Vice-Chancellor for Research and Technology, Mashhad University of Medical Sciences (Grant NO.: 980632; Ethical code: IR.MUMS.MEDICAL.REC.1398.653) and Ferdowsi University of Mashhad, Mashhad, Iran.
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Yasaman Abolhassani: Methodology, Investigation, Software, Analysis and interpretation of data, Writing- Original Draft. Sara Mirzaei: Methodology & Investigation. Masoud Nejabat: Writing – Review & Editing. Seyedehsaba Talebian: Methodology & Investigation. Hamid Gholamhosseinian: Radiotherapy. Mehrdad Iranshahi: AUR Synthesis. Fatemeh B. Rassouli, and Khadijeh Jamialahmadi: Supervision, Conceptualization and Design, Funding Acquisition.
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Abolhassani, Y., Mirzaei, S., Nejabat, M. et al. 7-Geranyloxcycoumarin enhances radio sensitivity in human prostate cancer cells. Mol Biol Rep 50, 5709–5717 (2023). https://doi.org/10.1007/s11033-023-08439-9
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DOI: https://doi.org/10.1007/s11033-023-08439-9