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Analysis of genetic diversity and population structure of oilseed crop noug (Guizotia abyssinica) accessions collected from Ethiopia

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Abstract

Background

Noug is an Ethiopian indigenous oilseed crop cultivated primarily for its oil and various economic importance. Evaluating the extent of genetic diversity within and among populations is one of the most important steps in breeding and conservation measures. Thus, this study aimed to uncover the extent of genetic diversity and population structure of noug accessions collected from different regions of Ethiopia using microsatellite markers.

Methods and results

A total of 161 accessions from fourteen regions of Ethiopia, including some from Eritrea using 13 microsatellite markers were analyzed. All the 13 microsatellite markers were polymorphic and highly informative with a mean PIC value of 0.82. The analysis generated a total of 158 alleles with a mean of 12.15 per locus. The overall mean of Shannon information index and heterozygosity/gene diversity were 1.57 and 0.74, respectively suggesting the presence of higher genetic diversity across the collection regions. AMOVA revealed that 96.06% of the total genetic variation was attributed to within populations while only 3.94% was attributed to among populations. Likewise, the dendrogram clustering, PCoA, and the model-based population structure analysis didn’t exactly corresponded the grouping of the genotypes according to their regions of origin.

Conclusion

The microsatellites used in the present study are highly informative and could be targeted for developing markers for future marker-assisted breeding. Genotypes collected from Shewa, Wollo, Gojjam, Tigray, and B/G showed a higher genetic diversity and private alleles as compared to other populations. Hence, these areas can be considered as hotspots which could help for the identification of genotypes that can be used in breeding programs as well as for the implementation of further conservation programs.

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Data availability

This is part of M.Sc. thesis for the first author and can be found on the University’s repository available at “http://etd.aau.edu.et/handle/123456789/27424”.

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Acknowledgements

The authors are grateful to the Ethiopian Biodiversity Institute (EBI) for the provision of noug-seed samples. The authors are also thankful to the Ethiopian Institute of Agricultural Research (EIAR) and Addis Ababa University, Institute of Biotechnology, for the financial support.

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Authors and Affiliations

  1. Ethiopian Institute of Agricultural Research, National Agricultural Biotechnology Research Center, Holeta, Ethiopia

    Motbaynor Terefe & Dejene Girma

  2. Institute of Biotechnology, Addis Ababa University, Addis Ababa, Ethiopia

    Genet Birmeta & Kassahun Tesfaye

  3. Department of Plant Breeding, Swedish University of Agricultural Sciences, Alnarp, Sweden

    Mulatu Geleta

  4. Ethiopian Biotechnology Institute, Addis Ababa, Ethiopia

    Kassahun Tesfaye

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  1. Motbaynor Terefe
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  2. Genet Birmeta
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Correspondence to Motbaynor Terefe.

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Supplementary file1 (XLSX 16 kb)

11033_2022_8005_MOESM2_ESM.jpg

Supplementary file2 Map showing noug sample collection regions (NB: all the sample areas, points, and boundaries are approximate and have nothing to do with political boundaries). The map was constructed using, ArcGIS_Desktop_1022_es_140418 (JPG 188 kb)

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Terefe, M., Birmeta, G., Girma, D. et al. Analysis of genetic diversity and population structure of oilseed crop noug (Guizotia abyssinica) accessions collected from Ethiopia. Mol Biol Rep 50, 43–55 (2023). https://doi.org/10.1007/s11033-022-08005-9

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