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Genotyping Helicobacter pylori and fgf7 gene expression in gastric cancer

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Abstract

Background

Helicobacter pylori as the causative agent of the most common chronic bacterial infectious disease in human still involves a range of clinical challenging complications. In this meantime, the survey of the interaction between H. pylori virulence genes expression and its consequences on gastric antral epithelial cells is Controversial. This study surveyed the correlations between H. pylori cag Pathogenicity Island and virulence factors genes with Fgf7 gene expression as an angiogenic factor in developing gastric cancer in gastric antral epithelial cells of patients with H. pylori infection.

Method

Gastric antral biopsy samples collected from patients out of exclusion criteria, including consumption of tobacco, alchohol and anti-H. pylori drugs, were categorized into gastric cancer (case group n:53) and gastritis (control group n:50) with and without H. pylori infection to detect changes in cDNA of fgf7 in gastric antral epithelial cells by using Real Time RT PCR. Extracted total RNA from gastric antral biopsy samples was used to synthesize cDNA for real time PCR. Furthermore, the cDNA of H. pylori cag Pathogenicity Island and other virulence factors genes were detected by using specific designed primers and simple PCR.

Results

Fgf7 gene expression revealed a significantly increase in gastric antral epithelial cells of gastric cancer and H. pylori-positive patients in contrast with gastritis and H. pylori-negative patients (p < 0.05). In the meanwhile, cag Pathogenicity Island and hopQ genotypes showed a positive correlation with Fgf7 gene expression (fold changes of cDNA) in gastric antral epithelial cells (p < 0.05).

Conclusion

This study revealed an obvious correlation between Fgf7 gene expression in gastric antral epithelial cells of patients with H. pylori carcinogenic genotypes infection and some host factors including age.

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Acknowledgements

This study is the result of Doctor Manouchehr Ahmadi Hedayati’s (Ph.D. of Medical Bacteriology) academic proposal. The authors thank Sanaz Ahmadi (MSc Medical Microbiology), Doctor Farshad Sheikhesmaeili (Gastroenterologist), Doctor Bijan Nouri (Biostatistician), Doctor Bahram Nikkhoo (Pathologist for clinicopathological assay), Doctor Roghayeh Ghadyani (Internal Medicine) and Zahra Jedi for supporting of sampling.

Funding

This study was supported and funded by the Kurdistan University of medical sciences with code number of IR.MUK.REC.1397/82.

Author information

Authors and Affiliations

  1. Liver and Digestive Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran

    Manouchehr Ahmadi Hedayati

  2. Department of Microbiology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran

    Manouchehr Ahmadi Hedayati & Delniya Khani

  3. Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran

    Delniya Khani

  4. Institute of Molecular and Cell Biology, Agency of Science, Technology and Research (A*STAR), Singapore, Singapore

    Hamed Bashiri

Authors
  1. Manouchehr Ahmadi Hedayati
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  2. Delniya Khani
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  3. Hamed Bashiri
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Contributions

MAH performed all the study steps, including designing, statistical analysis, Real-Time PCR, and corresponding author.

Corresponding author

Correspondence to Manouchehr Ahmadi Hedayati.

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Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

The ethical committee code number, IR.MUK.REC.1397/82, was provided and approved by the Kurdistan University of medical sciences. All experiments were performed according to the Helsinki guidelines.

Consent for publication

The institutional review board has approved the study as no published patients’ names were involved in the research project.

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Hedayati, M.A., Khani, D. & Bashiri, H. Genotyping Helicobacter pylori and fgf7 gene expression in gastric cancer. Mol Biol Rep 49, 8827–8834 (2022). https://doi.org/10.1007/s11033-022-07732-3

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  • DOI: https://doi.org/10.1007/s11033-022-07732-3

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