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CRISPR-Cas9 based stress tolerance: New hope for abiotic stress tolerance in chickpea (Cicer arietinum)

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Abstract

Plants are subjected to biotic and abiotic stresses regularly, which irreparably harm agricultural production. Eco-friendly and sustainable technology to deal with this challenge is to breed abiotic stress tolerant cultivars. To generate crop plants conferring resistance against stresses, conventional breeding was used in the past, but because of the complex heredity of abiotic stress tolerance traits, such techniques remain insufficient in making greater enhancement. Genome-engineering based on CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats-CRISPR associated protein9) has shown enormous potential in developing climate-resilient cultivars. Likewise, the development of chickpea transgenic lines by knockout of 4CL and REV7 genes exhibits drought tolerance which establishes a foundation for future studies in chickpea. In addition, the CRISPR-Cas9 system can boost yield potential under abiotic stress situations by producing non-transgenic plants having the required characteristics. This review article discusses the validation of gene function based on the CRISPR-Cas9 for the development of abiotic stress-tolerant crop plants, emphasizing the chickpea to open the new ventures of generating abiotic stress-tolerant chickpea varieties.

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Authors and Affiliations

  1. Soybean Research Institute, National Center for Soybean Improvement, Nanjing Agricultural University, 210095, Nanjing, China

    Muhammad Khuram Razzaq, Guangnan Xing & Junyi Gai

  2. Pulses Research Institute, AARI, Faisalabad, Pakistan

    Muhammad Akhter & Kaiser Latif Cheema

  3. Department of Plant Breeding and Genetics, University of Agriculture Faisalabad, Faisalabad, Pakistan

    Ramala Masood Ahmad

  4. Kohsar University Murree, Admin Block, Kashmir Point 47150, Murree, Pakistan

    Aiman Hina

  5. Department of Crop Science, Faculty of Agriculture, Food and Consumer Sciences, University for Development Studies, Tamale, Ghana

    Benjamin Karikari

  6. National Institute for Biotechnology and Genetic Engineering, Faisalabad, Pakistan

    Ghulam Raza

  7. Department of Microbiology, Government College University, Faisalabad, Pakistan

    Mohsin Khurshid

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Razzaq, M.K., Akhter, M., Ahmad, R.M. et al. CRISPR-Cas9 based stress tolerance: New hope for abiotic stress tolerance in chickpea (Cicer arietinum). Mol Biol Rep 49, 8977–8985 (2022). https://doi.org/10.1007/s11033-022-07391-4

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