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Expression of matrix metalloproteinases and tissue inhibitors of metalloproteinases in the hippocampus of lithium-pilocarpine-induced acute epileptic rats

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Abstract

Background

Epilepsy is characterised by abnormal neuronal discharges, including aberrant expression of extracellular matrix (ECM) components and synaptic plasticity stabilisation. Matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) interact to remodel the ECM in the central nervous system (CNS), to modulate synaptic plasticity in epileptogenesis.

Methods and Results

In the present study, the expression of MMP activators (tPA and uPA), 10 MMPs, and 3 TIMPs was detected by western blot analysis and quantitative polymerase chain reaction (RT-qPCR) to assess their potential pathogenetic role in the epileptogenesis in the hippocampus of lithium-pilocarpine hydrochloride-induced epileptic rats. Our results showed that The expression of MMP7 and MMP14 was impeded in the hippocampus of lithium-pilocarpine-induced acute epileptic rats compared with that in controls. The transcriptional level of tPA was enhanced on day 1 post-seizure in the hippocampus, while the levels of several MMPs and TIMPs did not change on days 1 and 3 post-seizure compared with that in controls.

Conclusions

The expression of MMPs and TIMPs reflects a novel feature of epileptogenesis and may offer new perspectives for future therapeutic interventions.

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Data availability statement

All data generated or analysed during this study are included in this published article.

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Funding

This work was funded by grants from the Key R&D Plan Project of Ningxia (2019BEB04041), National Natural Science Foundation of China (81960232 and 82001282), Youth Talents Supporting Program of Ningxia Medical University and Ningxia (XT2019018 and TJGC2019081), Natural Science Foundation of Ningxia (2018A0597).

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    Authors and Affiliations

    1. Department of Neurosurgery, General Hospital of Ningxia Medical University, 804 Shengli Street, Yinchuan, 750004, Ningxia, China

      Ding Wan, Qi Huang & Tao Sun

    2. School of Clinical Medicine, Ningxia Medical University, 750004, Yinchuan, China

      Jia Ren, Chen Zhang, Le Chen & Xueyao Su

    3. Ningxia Key Laboratory of Cerebrocranial Diseases, Department of Anatomy, Ningxia Medical University, 750004, Yinchuan, China

      Jianguo Niu

    4. Ningxia Key Laboratory of Cerebrocranial Diseases, Ningxia Medical University, 1160 Shengli Street, Yinchuan, 750004, Ningxia, China

      Ding Wan, Lu Yang, Haiyue Huang, Tao Sun & Peng Wang

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    1. Ding Wan
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    Contributions

    DW, and PW conceived and designed the experiments. DW, LY, CZ, LC, and XYS, performed experiments. JR, HYH, and QH, analysed the data and collected the references. DW and PW wrote the first draft; JGN, TS and PW checked and revised the draft. All authors approved the submission of this manuscript to be published.

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    Correspondence to Tao Sun or Peng Wang.

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    The authors declare no conflict of interest related to this work.

    Research involving animals and all protocols and procedures were approved by the National Institutes of Health and the animal welfare committee of Ningxia Medical University (Ethics Approval Number: 2019 − 151, Ningxia, China).

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    Ding Wan and Lu Yang contributed equally to this article.

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    Cite this article

    Wan, D., Yang, L., Ren, J. et al. Expression of matrix metalloproteinases and tissue inhibitors of metalloproteinases in the hippocampus of lithium-pilocarpine-induced acute epileptic rats. Mol Biol Rep 49, 5805–5810 (2022). https://doi.org/10.1007/s11033-022-07277-5

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    • DOI: https://doi.org/10.1007/s11033-022-07277-5

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