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Upregulation of TGF-β type II receptor in high glucose-induced vascular smooth muscle cells

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Abstract

Background

Mortality in patients with diabetes mellitus is estimated above 65% due to cardiovascular diseases. The aim of study was to investigate the effects of high-glucose conditions on TGF-β type II receptor (TGFBR2) expression levels, cell viability, and migration rate in vascular smooth muscle cells (VSMCs).

Methods

VSMCs were incubated in 30 mM and 50 mM of glucose for 24 h, 48 h, and 72 h periods. The gene and protein expression levels were investigated by Real-time qRT-PCR and western blotting techniques, respectively. The cell viability was evaluated by MTT assay. VSMC migration rate was also studied by wound healing assay.

Results

The TGFBR2 gene and protein expression levels were significantly upregulated in all the groups treated with glucose in 24 h, 48 h, and 72 h periods. The cell viability was not significantly affected in values of 30 mM and 50 mM of glucose. The increase of migration rate of VSMCs was not significant.

Conclusion

The results suggested the increased expression levels of TGFBR2 in the response to high glucose conditions may modulate the cellular events through the signaling pathway network in VSMCs.

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Data availability

The data sets used and/or analyzed during this study are available from the corresponding author on request.

Abbreviations

CVD:

Cardiovascular disease

VSMCs:

Vascular smooth muscle cells

AGEs:

Advanced glycation end-products

TGF-β:

Transforming growth factor-beta

TGFBR2:

TGF-β type II receptor

SMC:

Smooth muscle cells

DMEM:

Dulbecco’s modified eagle medium

FBS:

Fetal bovine serum

cDNA:

Complementary DNA

PVDF:

Polyvinylidene difluoride

TBST:

Tris-buffered saline with 0.1% Tween®20

ECL:

Enhanced chemiluminescence

PMSF:

Phenylmethylsulfonyl fluoride

PBS:

Phosphate-buffered saline

CMR:

Cellular migration rate

T2D:

Type 2 diabetes

H-RPE:

Human retinal pigment epithelium

MAPKs:

Mitogen-activated protein kinases

ERK:

Extracellular-signal-regulated kinase

JNK:

Jun N-terminal kinase

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Acknowledgements

No.

Funding

The study is supported by Iran University of Medical Sciences, Grant No. 14440.

Author information

Authors and Affiliations

  1. Clinical Biochemistry Department, Faculty of Medical Sciences, Iran University of Medical Sciences, Tehran, Iran

    Ghasem Ghasempour, Ali Akbar Soleimani & Mohammad Najafi

  2. Molecular and Cellular Research Center, Faculty of Medical Sciences, Iran University of Medical Sciences, Tehran, Iran

    Mohammad Najafi

  3. Microbial Biotechnology Research Center, Iran University of Medical Sciences, Tehran, Iran

    Mohammad Najafi

  4. Student Research Committee, Faculty of Medical Sciences, Iran University of Medical Sciences, Tehran, Iran

    Ghasem Ghasempour

  5. Clinical Biochemistry Department, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

    Asghar Mohammadi

  6. Clinical Biochemistry Department, Faculty of Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran

    Fahimeh Zamani-Garmsiri

Authors
  1. Ghasem Ghasempour
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  2. Asghar Mohammadi
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  3. Fahimeh Zamani-Garmsiri
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  4. Ali Akbar Soleimani
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Contributions

MN designed research; GG, FZG, AAS and AM performed experiments; MN and GG analyzed data; MN and GG wrote the manuscript. All authors reviewed and approved the manuscript.

Corresponding author

Correspondence to Mohammad Najafi.

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The authors declare no conflict of interest, financial or otherwise.

Ethical approval

The Ethical Committee of Iran University of Medical Sciences, Tehran, Iran, approves the study (14440).

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Ghasempour, G., Mohammadi, A., Zamani-Garmsiri, F. et al. Upregulation of TGF-β type II receptor in high glucose-induced vascular smooth muscle cells. Mol Biol Rep 49, 2869–2875 (2022). https://doi.org/10.1007/s11033-021-07100-7

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  • DOI: https://doi.org/10.1007/s11033-021-07100-7

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