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Seasonal expression of extracellular signal regulated kinases in the colon of wild ground squirrels (Spermophilus dauricus)

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Abstract

Background

The purpose of the experiment was to explore the localization and seasonal expression of extracellular signal regulated kinase (ERK) in the colonic tissue of wild ground squirrels (Spermophilus dauricus).

Methods and results

Hematoxylin–eosin staining, immunohistochemistry, real-time quantitative PCR and Western blotting were used in this experiment. The histological results showed that the diameter of the colon lumen enlarged and the number of glandular cells increased in the non-breeding season. It was found in the immunochemical results that both ERK1/2 and pERK1/2 were expressed in the cytoplasm of goblet cells and intestinal epithelial cells, while pERK1/2 was also expressed in the nucleus of them. The immune localization of both was more obvious in the non-breeding season, especially in intestinal epithelial cells. Real-time quantitative PCR and Western blotting showed that ERK1/2 and pERK1/2 were seasonally highly expressed in the non-breeding season.

Conclusions

The expression of ERK1/2 and pERK1/2 was seasonal changes and had significant increases in the non-breeding season. This study revealed that ERK1/2 had potential roles in the colon to the adaptation of seasonal changes in wild ground squirrels.

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Data availability

All data generated or analyzed during this study are included in this published article.

Code availability

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Funding

This research work is supported by the Fundamental Research Funds for the Central Universities (2018ZY21), and the National Training Program of Innovation and Entrepreneurship for Undergraduates (S202010022079, G202010022072).

Author information

Authors and Affiliations

  1. College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, People’s Republic of China

    Yue Song, Xiaoying Yang, Xueying Zhang, Jueyu Zhu, Fuli Gao, Haolin Zhang, Yingying Han, Qiang Weng & Zhengrong Yuan

  2. School of Information Science and Technology, Beijing Forestry University, Beijing, 100083, People’s Republic of China

    Yixin Chen

Authors
  1. Yue Song
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  2. Xiaoying Yang
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  3. Xueying Zhang
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  4. Jueyu Zhu
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  7. Haolin Zhang
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Contributions

YS, XY: Participated in sample collection, Performing the experiments, Assisted with all experiments, Analyzing the data, Drafting the manuscript. XZ, JZ: Participated in sample collection, Performing the experiments. YC: Participated in sample collection. FG, HZ: Assisted with all experiments, Helped revising the manuscript. YH: Helped revising the manuscript. QW, ZY*: Designed, Supervised the study, Revised manuscript.

Corresponding author

Correspondence to Zhengrong Yuan.

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Conflict of interest

The authors declare that they have no competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Ethical approval

All animal procedures were approved by the Policy on the Care and Use of Animals by the Ethical Committee, Beijing Forestry University and the Department of Agriculture of Hebei Province, China (JNZF11/2007).

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Cite this article

Song, Y., Yang, X., Zhang, X. et al. Seasonal expression of extracellular signal regulated kinases in the colon of wild ground squirrels (Spermophilus dauricus). Mol Biol Rep 49, 2209–2215 (2022). https://doi.org/10.1007/s11033-021-07042-0

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  • DOI: https://doi.org/10.1007/s11033-021-07042-0

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