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First isolation and molecular characterization of pseudorabies virus detected in Turkey

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Abstract

Background

Pigs are the main host species for the pseudorabies virus. It causes fatal encephalitis in many species, including humans. This article aims to report the first clinical case of pseudorabies as well as isolation and molecular characterization of the virus from a hunting dog in Bursa province, Turkey.

Methods and results

The dog shows clinical signs including pruritus and neurological signs such as stumbling and inability to stand up compatible with pseudorabies. The virus isolates were obtained from the supernatant of fresh tissue samples from the cerebellum, cornu ammonis, spleen, salivary gland, conjunctival swab, serum, and PBMC samples. The glycoprotein C region is targeted for viral DNA amplification. Pseudorabies virus genome detected both in fresh tissues and supernatants of third passage on Vero cells. The number of PCR positive samples was dramatically increased after cell culture inoculations. Genome sequencing of strain Bursa-10303, which was isolated from a non-endemic area, identified it to belong to clade A.

Conclusions

This study confirms the possible presence of pseudorabies infection in the wildlife reservoirs in Turkey. Future studies may clarify the importance of the infection in Turkey region, where there is no prevalent pig production.

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Acknowledgements

The authors thank veterinarian Dr. Evren Bulgaç for presenting the case and providing the samples.

Funding

Not applicable.

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Authors and Affiliations

  1. Department of Virology, Faculty of Veterinary Medicine, Bursa Uludag University, 16059, Bursa, Turkey

    Gizem Aytogu, Eda B. Toker, Berfin Kadiroglu, Ozer Ates & Kadir Yesilbag

  2. Department of Pathology, Faculty of Veterinary Medicine, Bursa Uludag University, 16059, Bursa, Turkey

    Ozkan Yavas & Musa Ozgur Ozyigit

Authors
  1. Gizem Aytogu
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  2. Eda B. Toker
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  3. Ozkan Yavas
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  4. Berfin Kadiroglu
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  5. Ozer Ates
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  6. Musa Ozgur Ozyigit
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  7. Kadir Yesilbag
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Corresponding author

Correspondence to Kadir Yesilbag.

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Conflict of interest

The authors declare that they have no conflicts of interest.

Ethical approval

This study was approved by the Bursa Uludag University Experimental Animals Local Ethics Committee in accordance with ethical guidelines for the Care and Use of Laboratory Animals (approval number: HADYEK; 2021–05/01).

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Aytogu, G., Toker, E.B., Yavas, O. et al. First isolation and molecular characterization of pseudorabies virus detected in Turkey. Mol Biol Rep 49, 1679–1686 (2022). https://doi.org/10.1007/s11033-021-06974-x

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  • DOI: https://doi.org/10.1007/s11033-021-06974-x

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