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Dietary uptake of Salvia macilenta extract improves Nrf2 antioxidant signaling pathway and diminishes inflammation and apoptosis in amyloid beta-induced rats

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Abstract

Background

Studies showed the protective role of Salvia in traditional medicine against neurodegenerative diseases. Salvia macilenta is one of the potent antioxidant herbs among Salvia species against oxidative stress. In the current study, the effect of oral administration of S. macilenta in the antioxidant, anti-inflammatory activities of Aβ-injected male albino Wistar rats was determined.

Methods

Rats were received S. macilenta (50 mg/kg/day) orally, for ten successive days and then some of them received Aβ (10 ng/µl) in their hippocampus (CA1 region). Proteins involved in antioxidant defense system and inflammatory signaling pathways in the hippocampus and prefrontal cortex were evaluated using Western blotting technique. To study apoptosis, Western blotting technique and histological staining were used. Catalase activity, glutathione peroxidase (GSH) and nitric oxide levels were measured.

Results

Results demonstrated that S. macilenta increased Nrf2 protein level and decreased TNFα and IL-6 protein level in Aβ-injected rats compared to the Aβ-injected group in the hippocampus and prefrontal cortex. Histological analysis showed pretreatment with S. macilenta decreased apoptosis levels in the hippocampus and prefrontal cortex, about 41 and 42%, compared to Aβ-injected rats, respectively. This study showed that catalase activity was changed in the S. macilenta + Aβ group compared to the Aβ-injected rats. Also, GSH level was increased in the S. macilenta + Aβ group compared to the Aβ-injected rat.

Conclusion

Orally treatment of S. macilenta extract in Aβ-injected rats could ameliorate protective pathways and, so, it can be one of the proposed dietary supplements for the prevention of Alzheimer’s disease and dementia.

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Acknowledgements

This work was supported by Tehran University of Medical Sciences (Grant No. 1400-1-101-52633), Islamic Azad University Damghan Branch and Shahid Beheshti University of Medical Sciences Funds.

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Authors and Affiliations

  1. Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran

    Shabnam Taheri & Hooman Shajiee

  2. Cognitive and Neuroscience Research Center (CNRC), Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

    Solmaz Khalifeh

  3. Department of Physiology, School of Medicine, Tehran University of Medical Sciences, 1417613151, Tehran, Iran

    Ghorbangol Ashabi

Authors
  1. Shabnam Taheri
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  2. Solmaz Khalifeh
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  3. Hooman Shajiee
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  4. Ghorbangol Ashabi
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Contributions

GA and SK: conceptualization, formal analysis, data curation, writing manuscript. ST: performing the experiment. HS: formal analysis.

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Correspondence to Hooman Shajiee or Ghorbangol Ashabi.

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Taheri, S., Khalifeh, S., Shajiee, H. et al. Dietary uptake of Salvia macilenta extract improves Nrf2 antioxidant signaling pathway and diminishes inflammation and apoptosis in amyloid beta-induced rats. Mol Biol Rep 48, 7667–7676 (2021). https://doi.org/10.1007/s11033-021-06772-5

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  • DOI: https://doi.org/10.1007/s11033-021-06772-5

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