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Mitochondrial DNA markers reveal genetic connectivity among populations of Osteoglossiform fish Chitala chitala

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Abstract

Genetic diversity and population structure in Indian featherback fish, Chitala chitala (Hamilton, 1822) was investigated by combined analyses of two full mitochondrial genes, ATPase 6/8 and Cytochrome b. A total of 403 individuals, collected from 14 rivers yielded 61 haplotypes. Hierarchical partitioning analysis identified 19.01% variance ‘among’ and 80.99% variance ‘within groups and populations’. The mean coefficient of genetic differentiation (FST) was observed to be significant 0.26 (p < 0.05). Mantel tests rejected the hypothesis that genetic and geographic distances were correlated. The patterns of genetic differentiation, AMOVA and principal coordinate analyses indicated that natural populations were sub-structured and comprised of four genetic stocks of C. chitala in Indian rivers. The results also supported the higher resolution potential of concatenated gene sequences. The knowledge of genetic variation and divergence, from this study, can be utilized for its scientific conservation and management in the wild.

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Acknowledgments

This work has been carried out under the project scheme of the Indian Council for Agricultural Research, India entitled, “Outreach Activity on Fish Genetic Stocks Phase-II”. The authors are thankful to Director, ICAR-National Bureau of Fish Genetic Resources, Lucknow, India for providing the facilities needed for executing this work. The authors are also grateful to Sh Pradipta Paul, Dr. L.K Tyagi, Dr. Santosh Kumar, Sh Kantharajan G, Dr. Achal Singh, and Sh Rajkumar for their help in sampling and geo-referenced riverine map preparations. Sh R.S. Sah and Sh Rajesh Kumar are thankfully acknowledged for their excellent technical assistance.

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Authors and Affiliations

  1. National Bureau of Fish Genetic Resources, Lucknow, India

    Nimisha Dutta, Rajeev Kumar Singh, Abhinav Pathak, Vindhya Mohindra, Sangeeta Mandal & Kuldeep Kumar Lal

  2. Amity Institute of Biotechnology, Amity University, Lucknow Campus, Lucknow, India

    Nimisha Dutta & Gurjeet Kaur

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  1. Nimisha Dutta
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Correspondence to Rajeev Kumar Singh.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. The methodologies were approved by the Institutional Animal Ethical Committee (IAEC), ICAR-NBFGR, Lucknow, India vide No. G/CPCSEA/IAEC/2015/2 dated 27 October, 2015.

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Dutta, N., Singh, R.K., Pathak, A. et al. Mitochondrial DNA markers reveal genetic connectivity among populations of Osteoglossiform fish Chitala chitala. Mol Biol Rep 47, 8579–8592 (2020). https://doi.org/10.1007/s11033-020-05901-w

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