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Ontogenetic and tissue-specific expression of gonadotropin-inhibitory hormone (GnIH) and its receptors in Catla catla

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Abstract

Gonadotropin-inhibitory hormone (GnIH) is an RFamide peptide, and its role in reproduction is well studied from fish to mammals, but very few reports are available about the function of GnIH during larval development. In this study, we examined the GnIH and GnIH receptors (GnIHRs) expression from embryogenesis to adult stage and tissue-specific expression in adult Catla catla using quantitative real-time (qRT) PCR. The qRT PCR analysis of GnIH mRNA during ontogenetic development showed the increasing trend from early developmental stages to the adult stage with the highest expression in 24 months fish. However, the expression of two GnIH receptors, GnIHR1 and GnIHR2 also increased from larval stages to the adults with a peak at 17 days post-hatching, while GnIHR3 showed the higher mRNA expression during embryogenesis and then decreasing gradually. Tissue distribution analysis of GnIH showed the highest mRNA expression of GnIH in the brain, followed by gonads of both the sexes. GnIHR1 and GnIHR2 were also highly expressed in the brain and gonads of both the sexes, while GnIHR3 showed the highest expression in gonads of both the sexes without any expression in the brain. These results suggest that the brain is the primary site of action for GnIH, GnIHR1 and GnIHR2, while gonads for GnIHR3.

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Acknowledgements

The authors are grateful to Director & Vice-Chancellor, ICAR-Central Institute of Fisheries Education, Mumbai, India for providing support and necessary facilities for carrying out this experiment.

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Authors and Affiliations

  1. Division of Fish Genetics and Biotechnology, ICAR-Central Institute of Fisheries Education, Versova, Mumbai, 400061, India

    Pravesh Kumar, K. S. Wisdom, Gulshan Kumar, Pathakota Gireesh-Babu, Sunil Kumar Nayak, N. S. Nagpure & Rupam Sharma

  2. Department of Aquaculture, College of Fisheries, Dr. Rajendra Prasad Central Agricultural University, Pusa, Bihar, India

    Pravesh Kumar

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  1. Pravesh Kumar
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Kumar, P., Wisdom, K.S., Kumar, G. et al. Ontogenetic and tissue-specific expression of gonadotropin-inhibitory hormone (GnIH) and its receptors in Catla catla. Mol Biol Rep 47, 3281–3290 (2020). https://doi.org/10.1007/s11033-020-05388-5

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