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Identification of human endogenous retrovirus transcripts in Hodgkin Lymphoma cells

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Abstract

During the last decades, the prognosis for patients with Hodgkin Lymphoma (HL) has been steadily improved. Nevertheless, new and less toxic therapy strategies have to be developed especially for patients with advanced disease. The activation of human endogenous retroviruses (HERV) is suspected to occur in HL and therefore, HERV might represent interesting target structures. In order to identify transcribed HERV of the HERV-H and HERV-K families in HL we used a reverse transcription-polymerase chain reaction based cloning approach. In addition to unspliced HERV-H and HERV-K transcripts, we detected spliced HERV-K transcripts that matched genomic sequences with the expected splicing-donor and splicing-acceptor sites. Of particular interest was the expression of HERV-K18 related transcripts at the CD48 locus. Our data indicate transcriptional activity of several HERV loci in HL cells.

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Acknowledgements

We thank Ines Volkmer for technical assistance and Dixie Mager for helpful advice.

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Authors and Affiliations

  1. University Clinic and Outpatient Clinic for Pediatrics I, Martin Luther University Halle-Wittenberg, Ernst-Grube-Str. 40, 06120, Halle, Germany

    Marie Barth & Martin Sebastian Staege

  2. Department of Drug Design and Target Validation, Fraunhofer Institute for Cell Therapy and Immunology, 06120, Halle, Germany

    Victoria Gröger & Holger Cynis

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  1. Marie Barth
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  2. Victoria Gröger
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  4. Martin Sebastian Staege
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MB, VC and HC performed experiments, MSS designed the study, all authors analyzed the data and wrote the paper.

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Correspondence to Martin Sebastian Staege.

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Barth, M., Gröger, V., Cynis, H. et al. Identification of human endogenous retrovirus transcripts in Hodgkin Lymphoma cells. Mol Biol Rep 46, 1885–1893 (2019). https://doi.org/10.1007/s11033-019-04640-x

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  • DOI: https://doi.org/10.1007/s11033-019-04640-x

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