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Proteomic changes in rice leaves grown under open field high temperature stress conditions

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Abstract

The interactive effect of temperature with other climatic and soil factors has profound influences on the growth and development of rice. The responses of rice to high temperatures under field conditions are more important than those under the controlled conditions. To understand the genes associated with high temperature stress response in general and tolerance in particular, the expression of all those genes associated with adaptation and tolerance in rice requires proteomic analysis. High temperature stress-tolerant cv. N22 was subjected to 28/18 °C (control) and 42/32 °C (high temperature stress) at flowering stage. The plants were grown in the field under the free air temperature increment condition. The proteomic changes in rice leaves due to high temperature stress were discussed. The proteomes of leaves had about 3000 protein spots, reproducibly detected on 2-dimensional electrophoretic gels with 573 proteins differentially expressed between the control and the high temperature treatments. Putative physiological functions suggested five categories such as growth (15.4 %), heat shock proteins (7.7 %), regulatory proteins (26.9 %), redox homeostasis proteins (11.5 %) and energy and metabolism (38.5 %) related proteins. The results of the present study suggest that cv. N22, an agronomically recognized temperature tolerant rice cultivar copes with high temperature stress in a complex manner. Several functional proteins play important roles in its responses. The predicted climate change events necessitate more studies using this cultivar under different simulated ecological conditions to identify proteomic changes and the associated genes to be used as biomarkers and to gain a better understanding on the biochemical pathways involved in tolerance.

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Acknowledgments

This research was supported by grants from the Science and Engineering Research Council (SERC), Department of Science and Technology (DST), Government of India, for the project on “High temperature stress response and associated changes in growth and yield of rice (Oryza sativa L),” to P. K. and B. R. We acknowledge the facilities and support provided by the Director, ICAR-IARI, New Delhi and Project Director, ICAR-NRCPB, New Delhi. S. Das is grateful to DST for the support of Junior Research Fellowship. None of the co-authors have a conflict of interest in submitting this manuscript.

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  1. P. Krishnan

    Present address: Laboratory of Biophysics, Division of Agricultural Physics, Indian Council of Agricultural Research (ICAR)-Indian Agricultural Research Institute (IARI), New Delhi, 110012, India

Authors and Affiliations

  1. Laboratory of Biophysics, Division of Agricultural Physics, Indian Council of Agricultural Research (ICAR)-Indian Agricultural Research Institute (IARI), New Delhi, 110012, India

    Smruti Das & Ritesh Kumar

  2. Division of Microbiology, ICAR-IARI, New Delhi, 110012, India

    B. Ramakrishnan

  3. ICAR-National Research Centre for Plant Biotechnology (NRCPB), New Delhi, 110012, India

    Vagish Mishra & N. K. Singh

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  1. Smruti Das
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Das, S., Krishnan, P., Mishra, V. et al. Proteomic changes in rice leaves grown under open field high temperature stress conditions. Mol Biol Rep 42, 1545–1558 (2015). https://doi.org/10.1007/s11033-015-3923-5

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