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Fine mapping of wmv1551, a resistance gene to Watermelon mosaic virus in melon

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Abstract

Recessive resistance to Watermelon mosaic virus (WMV) in melon has previously been reported in the African accession TGR-1551. Using a population of recombinant inbred lines (RIL), derived from a cross between TGR-1551 and the susceptible Spanish cultivar ‘Bola de Oro’ (BO), a major quantitative trait locus (QTL) controlling the resistance was previously mapped to a region of approximately 760 kb in chromosome 11. Minor QTLs were also reported with lower effects, dependent on the environmental conditions. A genotyping by sequencing (GBS) analysis of the RIL population has provided new information that allowed the better location of the major QTL in chromosome 11. Moreover, three minor QTLs in chromosomes 4, 5, and 6 were identified. Generations derived from the RIL population were subsequently phenotyped for resistance and genotyped with SNP markers to fine map the resistance derived from TGR-1551. The results obtained have allowed to narrow the position of the resistance gene on chromosome 11, designated as wmv1551, to a 141-kb region, and the confirmation of a minor QTL in chromosome 5. The effect of the minor QTL in chromosome 5 was significant in heterozygote plants for the introgression in chromosome 11. The SNP markers linked to both QTLs will be useful in breeding programs aimed at the introgression of WMV resistance derived from TGR-1551. Future work will be directed to identifying the resistance gene, wmv1551, in the candidate region on chromosome 11.

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Acknowledgments

The authors would like to thank R. Camero, I. Díaz, E. Martínez, G. Perpiñá, M. López, V. Aragonés, and T. Cordero for their technical support in field assays.

Funding

This study was partially supported by the Spanish Ministerio de Economía y Competitividad grants AGL2014-53398-C2 (1-R and 2-R), by the Spanish Ministerio de Ciencia, Innovación y Universidades grants AGL2017-85563-C2 (1-R and 2-R) and BIO2017-83184-R, and by the PROMETEO project 2017/078 (to promote excellence groups) by the Conselleria d’Educació, Investigació, Cultura i Esports (Generalitat Valenciana).

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Authors and Affiliations

  1. Instituto de Conservación y Mejora de la Agrodiversidad (COMAV), Universitat Politècnica de València, Camino de Vera, 46022, Valencia, Spain

    Ana Pérez-de-Castro, Cristina Esteras & Belén Picó

  2. Grupo de Virología, Instituto Agroforestal Mediterráneo, Universitat Politècnica de València, Camino de Vera, 46022, Valencia, Spain

    Ana Alfaro-Fernández

  3. Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas-Universitat Politècnica de València, 46022, Valencia, Spain

    José-Antonio Daròs & Antonio José Monforte

  4. Instituto de Hortofruticultura Subtropical y Mediterránea ‘La Mayora’ (IHSM, UMA-CSIC), Algarrobo-Costa, 29760, Málaga, Spain

    María Luisa Gómez-Guillamón

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  1. Ana Pérez-de-Castro
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  2. Cristina Esteras
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  3. Ana Alfaro-Fernández
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  4. José-Antonio Daròs
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Correspondence to Ana Pérez-de-Castro.

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Pérez-de-Castro, A., Esteras, C., Alfaro-Fernández, A. et al. Fine mapping of wmv1551, a resistance gene to Watermelon mosaic virus in melon. Mol Breeding 39, 93 (2019). https://doi.org/10.1007/s11032-019-0998-z

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