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Salt tolerance conferred by expression of a global regulator IrrE from Deinococcus radiodurans in oilseed rape

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Abstract

As salinity is a major threat to sustainable agriculture worldwide, cultivation of salt-tolerant crops becomes increasingly important. IrrE acts as a global regulator and a general switch for stress resistance in Deinococcus radiodurans. In this study, to determine whether the irrE gene can improve the salt tolerance of Brassica napus, we introduced the irrE gene into B. napus by the Agrobacterium tumefaciens-mediated transformation method. Forty-two independent transgenic plants were regenerated. Polymerase chain reaction (PCR) analyses confirmed that the irrE gene had integrated into the plant genome. Northern as well as Western blot analyses revealed that the transgene was expressed at various levels in transgenic plants. Analysis for the T1 progenies derived from four independent transformants showed that irrE had enhanced the salt tolerance of T1 in the presence of 350 mM NaCl. Furthermore, under salt stress, transgenic plants accumulated more compatible solutes (proline) and a lower level of malondialdehyde (MDA), and they had higher activities of catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD). However, agronomic traits were not affected by irrE gene overexpression in the transgenic B. napus plants. This study indicates that the irrE gene can improve the salt tolerance of B. napus and represents a promising candidate for the development of crops with enhanced salt tolerance by genetic engineering.

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Acknowledgments

This work was funded by the 973 Project in China (2013CB733903) and China Transgenic Program (2014ZX0801201B).

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    Authors and Affiliations

    1. Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

      Jin Wang & Min Lin

    2. Life Science and Engineering College, Southwest University of Science and Technology, Mianyang, 621000, Sichuan, China

      Cui Guo, Qilin Dai & Bin Feng

    3. School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai, 200030, China

      Kaijing Zuo

    Authors
    1. Jin Wang
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    2. Cui Guo
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    3. Qilin Dai
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    4. Bin Feng
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    5. Kaijing Zuo
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    6. Min Lin
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    Corresponding authors

    Correspondence to Kaijing Zuo or Min Lin.

    Additional information

    Jin Wang, Cui Guo, and Qilin Dai have contributed equally to this paper.

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    11032_2016_486_MOESM1_ESM.jpg

    Fig. S1 Generating transgenic irrE oilseed rape plants. A Schematic diagram of the irrE expression cassette in the binary vector pBI121. The transgene was inserted between the CaMV35S promoter (35S) and NOS terminator (NOST); the NPTII marker is flanked by the NOS promoter (NOSP) and terminator (NOS-Ter). B–C The formation of the callus during transformation. D The screening of the explants and regenerating of transgenic B. napus plants. E Transferring the regenerated plants into soil (JPEG 11002 kb)

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    Wang, J., Guo, C., Dai, Q. et al. Salt tolerance conferred by expression of a global regulator IrrE from Deinococcus radiodurans in oilseed rape. Mol Breeding 36, 88 (2016). https://doi.org/10.1007/s11032-016-0486-7

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