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Organosulfur compound protects against memory decline induced by scopolamine through modulation of oxidative stress and Na+/K+ ATPase activity in mice

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Abstract

The present study investigated the possible effect of BMMS in protecting against memory impairment in an Alzheimer’s disease model induced by scopolamine in mice. Another objective was to evaluate the involvement of oxidative stress and Na+/K+ ATPase activity in cerebral cortex and hippocampus of mice. Male Swiss mice were divided into four groups: groups I and III received canola oil (10 ml/kg, intragastrically (i.g.)), while groups II and IV received BMMS (10 mg/kg, i.g.). Thirty minutes after treatments, groups III and IV received scopolamine (1 mg/kg, intraperitoneal (i.p.)), while groups I and II received saline (5 ml/kg, i.p.). Behavioral tests were performed thirty minutes after scopolamine or saline injection. Cerebral cortex and hippocampus were removed to determine the thiobarbituric acid reactive species (TBARS) levels, non-protein thiols (NPSH) content, catalase (CAT) and Na+/K+ ATPase activities. The results showed that BMMS pretreatment protected against the reduction in alternation and latency time induced by scopolamine in the Y-maze test and step-down inhibitory avoidance, respectively. In the Barnes maze, the latency to find the escape box and the number of holes visited were attenuated by BMMS. Locomotor and exploratory activities were similar in all groups. BMMS pretreatment protected against the increase in the TBARS levels, NPSH content and CAT activity, as well as the inhibition on the Na+/K+ ATPase activity caused by scopolamine in the cerebral cortex. In the hippocampus, no significant difference was observed. In conclusion, the present study revealed that BMMS protected against the impairment of retrieval of short-term and long-term memories caused by scopolamine in mice. Moreover, antioxidant effect and protection on the Na+/K+ ATPase activity are involved in the effect of compound against memory impairment in AD model induced by scopolamine.

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Acknowledgements

We gratefully acknowledge UFPel, UNIFRA, FAPERGS (research grants #16/2551-0000526-5 – PRONUPEQ and # 10/0005-1 – PRONEX), CAPES and CNPq for the financial support. C.C.S, C.R.J. and C.L. are recipients of CNPq fellowship. M.P.P. is recipients of CAPES fellowship.

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Authors and Affiliations

  1. Programa de Pós-Graduação em Nanociências, Centro de Ciências Tecnológicas, Centro Universitário Franciscano, Santa Maria, RS, CEP 97010-032, Brazil

    Fernanda D. da Silva & Francine R. Ianiski

  2. Programa de Pós-Graduação em Bioquímica e Bioprospecção, Laboratório de Pesquisa em Farmacologia Bioquímica (LaFarBio), Grupo de pesquisa em Neurobiotecnologia (GPN), Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas, Pelotas, Campus Capão do Leão, Pelotas, RS, CEP 96010-900, Brazil

    Mikaela P. Pinz, Renata L. de Oliveira, Karline C. Rodrigues, Ethel A. Wilhelm & Cristiane Luchese

  3. Programa de Pós-Graduação em Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS, CEP 97105-900, Brazil

    Mariana M. Bassaco & Claudio C. Silveira

  4. Laboratório de Avaliações Farmacológicas e Toxicológicas Aplicadas às Moléculas Bioativas, LaftamBio Pampa, Universidade Federal do Pampa, Itaqui, RS, CEP 97650-000, Brazil

    Cristiano R. Jesse

  5. Universidade Regional Integrada, Campus Erechim, RS, CEP 99700-000, Brazil

    Silvane S. Roman

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  1. Fernanda D. da Silva
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  2. Mikaela P. Pinz
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  3. Renata L. de Oliveira
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  5. Francine R. Ianiski
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  6. Mariana M. Bassaco
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  7. Claudio C. Silveira
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  8. Cristiano R. Jesse
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  10. Ethel A. Wilhelm
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Correspondence to Ethel A. Wilhelm or Cristiane Luchese.

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All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted (number 007/2011).

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da Silva, F.D., Pinz, M.P., de Oliveira, R.L. et al. Organosulfur compound protects against memory decline induced by scopolamine through modulation of oxidative stress and Na+/K+ ATPase activity in mice. Metab Brain Dis 32, 1819–1828 (2017). https://doi.org/10.1007/s11011-017-0067-4

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