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Isolation methods, proliferation, and adipogenic differentiation of adipose-derived stem cells from different fat depots in bovines

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Abstract

The adipose-derived stem cells (ASCs) are a valuable resource for regenerative medicine and essential materials for research in fat deposition. However, the isolation procedure of ASCs has not been standardized and needs to be harmonized; differences in proliferation and adipogenic differentiation of ASCs obtained from different fat depots have not been well characterized. In the present study, we compared the efficiency of ASCs isolation by enzymatic treatment and explant culture methods and the proliferation ability and adipogenic differentiation potential of ASCs isolated from subcutaneous and visceral fat depots. The explant culture method was simple and with no need for expensive enzymes while the enzymatic treatment method was complex, time consuming and costly. By the explant culture method, a larger number of ASCs were isolated from subcutaneous and visceral fat depots. By contrast, fewer ASCs were obtained by the enzymatic treatment method, especially from visceral adipose. ASCs isolated by the explant culture method performed well in cell proliferation and adipogenic differentiation, though they were slightly lower than those by the enzymatic treatment method. ASCs isolated from visceral depot demonstrated higher proliferation ability and adipogenic differentiation potential. In total, the explant culture method is simpler, more efficient, and lower cost than the enzymatic treatment method for ASCs isolation; compared with visceral adipose, subcutaneous adipose is easier to isolate ASCs; however, the visceral ASCs are superior to subcutaneous ASCs in proliferation and adipogenic differentiation.

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All data supporting the findings of this study are available from the corresponding author upon reasonable request.

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Acknowledgements

We would like to thank the Guangxi Bagui Scholar Program, Innovation Project of Guangxi Graduate Education and Bama County Program for Talents in Science and Technology.

Funding

This study was supported by the National Natural Science Foundation of China (32060747 and U20A2051) and the Guangxi Natural Science Foundation (2020JJA130143 and 2021AC19014).

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Authors and Affiliations

  1. State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Guangxi University, Nanning, 530005, China

    Ruirui Zhu, Ye Feng, Ruirui Li, Deshun Shi & Jieping Huang

  2. Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning, 530001, China

    Kelong Wei

  3. School of Agriculture, Ningxia University, Ningxia, 750021, China

    Yun Ma

  4. Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Life Science and Engineering, Foshan University, Foshan, 528225, China

    Qingyou Liu

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  1. Ruirui Zhu
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  2. Ye Feng
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Contributions

JH, YM, and DS: concepts, design. RZ, YF, RL and KW, QL: material preparation. RZ, YF: experimental studies. RZ: validation, data analysis, software, and validation. JH: writing—reviewing and editing, funding acquisition, and supervision. All authors read and approved the final manuscript.

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Correspondence to Jieping Huang.

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All animal protocols were approved by the Animal Care Committee of the College of Animal Science and Technology, Guangxi University (Gxu-2021-050).

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Zhu, R., Feng, Y., Li, R. et al. Isolation methods, proliferation, and adipogenic differentiation of adipose-derived stem cells from different fat depots in bovines. Mol Cell Biochem 479, 643–652 (2024). https://doi.org/10.1007/s11010-023-04753-9

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