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Response of DNA damage genes in acrolein-treated lung adenocarcinoma cells

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Abstract

Acrolein is a α-β-unsaturated aldehyde and is toxic to human upon its exposure from the environment. Sources of exposure to acrolein can be from heating cooking oil, automobile exhaust, tobacco smoke, and plastic waste. Acrolein exposure to lung is a major concern because of its volatile nature and due to its presence in the urban atmospheric air. Acrolein being highly reactive forms DNA and protein adducts, thereby making the cells vulnerable to long-term damage. Such long-term effect can lead to high susceptibility towards malignant transformation as has been reported in cigarette smokers. The response of DNA damaging genes by acrolein can perhaps give an insight to the cause of damage in the DNA by acrolein. The aim of this study was to examine the response of the DNA damage responsive genes by acrolein in A549 lung adenocarcinoma cells. Acrolein treatment at IC50 concentration showed a robust response of the DNA repair genes but eventually failed to rescue the cells from undergoing apoptosis. The cells pretreated with acrolein and followed by growing the same cells in fresh medium in the absence of acrolein did not help the cells to proliferate. These results conclude that exposure to acrolein marks long-lasting damage to DNA, irrespective of the DNA repair response.

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Acknowledgements

This publication was supported in part by research infrastructure support from Grant Numbers RR0304518 and CO6 RR012537 from the National Center for Research Resources (NCRR). The author would like to extend thanks to Prof. Barbara E. Hayes for her continued support and encouragement during the course of this study.

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  1. Poonam Sarkar

    Present address: Department of Pediatrics, Texas Children’s Cancer Center, Baylor College of Medicine, 1102 Bates St. Feigin Center, Houston, TX, 77030, USA

Authors and Affiliations

  1. College of Pharmacy and Health Sciences, Texas Southern University, 3100 Cleburne Street, Houston, TX, 77004, USA

    Poonam Sarkar

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  1. Poonam Sarkar
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Correspondence to Poonam Sarkar.

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Sarkar, P. Response of DNA damage genes in acrolein-treated lung adenocarcinoma cells. Mol Cell Biochem 450, 187–198 (2019). https://doi.org/10.1007/s11010-018-3385-x

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  • DOI: https://doi.org/10.1007/s11010-018-3385-x

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