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Ecto-enzymes activities in splenic lymphocytes of mice experimentally infected by Trypanosoma cruzi and treated with specific avian immunoglobulins: an attempt to improve the immune response

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Abstract

The aim of this study was to evaluate the therapeutic efficacy of specific avian polyclonal antibodies (IgY) against Trypanosoma cruzi and their interaction with ecto-enzymes of the purinergic system (NTPDase and adenosine deaminase (ADA) activities) in splenic lymphocytes. For this, mice were divided into six groups: three non-infected (A, B, and C) and three infected (D, E, and F). The groups A and D were composed by negative and positive controls, respectively; while the groups B and E were treated prophylactically with IgY (50 mg/kg), and the groups C and F were treated therapeutically with IgY (50 mg/kg). Treatment with IgY reduced parasitemia on day 6 post-infection (PI) compared to the infected control group, but it was similar on day 8 PI. Moreover, infected and treated animals (the groups E and F) did not show neither amastigotes in the cardiac tissue nor cardiac lesions when compared to the positive control group (the group D). The E-NTPDase (ATP and ADP as substrate) and ADA activities in splenic lymphocytes increased significantly in the positive control group (the group D) compared to the negative control group (the group A). The therapeutic treatment of IgY (the group F) was able to prevent the increase of E-NTPDase and E-ADA activities compared to the positive control group (the group D), but this finding was not observed in animals that received the prophylactic treatment (the group E). The therapeutic treatment of IgY may be considered an interesting approach to improve the immune response of mice experimentally infected by T. cruzi.

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Authors and Affiliations

  1. Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil

    Thirssa H. Grando, Matheus D. Baldissera, Guilherme Do Carmo, Eduarda T. Santi, Pedro Henrique Doleski, Daniela B. R. Leal & Silvia G. Monteiro

  2. Department of Animal Science, Universidade do Estado de Santa Catarina (UDESC), Chapecó, SC, Brazil

    Lenita Moura Stefani & Aleksandro S. Da Silva

  3. Veterinary Pathology Laboratory, Instituto Federal Catarinense (IFC), Concórdia, SC, Brazil

    Ricardo E. Mendes

  4. Universidade Federal de Pelotas (UFPel), Pelotas, RS, Brazil

    Camila B. Oliveira

Authors
  1. Thirssa H. Grando
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  2. Matheus D. Baldissera
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  3. Guilherme Do Carmo
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  4. Camila B. Oliveira
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  5. Eduarda T. Santi
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  6. Pedro Henrique Doleski
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  7. Daniela B. R. Leal
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  8. Lenita Moura Stefani
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  9. Ricardo E. Mendes
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  10. Aleksandro S. Da Silva
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  11. Silvia G. Monteiro
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Corresponding author

Correspondence to Thirssa H. Grando.

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The authors declare that they have no conflict of interest.

Ethical approval

All procedures in this study were approved by the Animal Welfare Committee of Ethics in Animal Experimentation of the Universidade Federal de Santa Maria (UFSM), under protocol number 5699170915, following the guidelines established by Brazilian Society of Science in Laboratory Animals.

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Grando, T.H., Baldissera, M.D., Do Carmo, G. et al. Ecto-enzymes activities in splenic lymphocytes of mice experimentally infected by Trypanosoma cruzi and treated with specific avian immunoglobulins: an attempt to improve the immune response. Mol Cell Biochem 448, 9–15 (2018). https://doi.org/10.1007/s11010-018-3308-x

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  • DOI: https://doi.org/10.1007/s11010-018-3308-x

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