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Quercetin exerts an inhibitory effect on cellular bioenergetics of the B164A5 murine melanoma cell line

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Abstract

Modulation of mitochondrial bioenergetics and glycolysis in malignancies has recently emerged a potential chemotherapeutic strategy since numerous malignant cells have overcome inhibition of the glycolytic pathway by increasing mitochondrial ATP production. Quercetin is a flavonoid with antioxidant, antiangiogenic, and chemoprotective properties but the mitochondrial effects are less characterized. The present study was purported to assess the effects of quercetin on the bioenergetic profile of B164A5 murine melanoma cell line. The oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) were measured at 24, 48, and 72 h of treatment of B164A5 murine melanoma cells with increasing concentrations (25, 50, 100, and 150 µM) of quercetin using the extracellular flux analyzer Seahorse XF24e (Seahorse Agilent). Analysis of mitochondrial function was performed in the presence of the classic modulators of the electron transport chain: oligomycin, FCCP, and rotenone. 72-h treatment with quercetin induced a dose-dependent decrease of all OCR parameters (basal respiration, proton leak, ATP turnover, maximal respiration, reserve capacity) as well as of ECAR. At variance, 48-h treatment induced a decrease of OCR and ECAR when quercetin was applied at 50, 100, and 150 µM, while the 24-h treatment induced a decrease of bioenergetic parameters only for the highest concentrations (100 and 150 µM) of the compound. Our data clearly demonstrated that quercetin elicited dose-dependent inhibitory effect on examined parameters of cellular bioenergetics that was most potent at 72 h of treatment. Thereby quercetin, modulating both glycolytic and mitochondrial pathways for ATP production, might be an efficient approach in killing cancer cells.

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Acknowledgements

The research was funded by the university Grant PIII-C2-PCFI-2015/2016.

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Authors and Affiliations

  1. Department of Pathophysiology - Functional Sciences, Faculty of Medicine, “Victor Babeș” University of Medicine and Pharmacy of Timişoara, 2, Eftimie Murgu Sq., 300041, Timisoara, Romania

    Adrian Sturza, Sebastian Ancușa, Oana Duicu & Danina Muntean

  2. Department of Pharmacognosy, Faculty of Pharmacy, “Victor Babeș” University of Medicine and Pharmacy, Timisoara, Romania

    Ioana Pavel & Corina Danciu

  3. Department of Toxicology, Faculty of Pharmacy, “Victor Babeș” University of Medicine and Pharmacy, Timisoara, Romania

    Cristina Dehelean

  4. Center for Translational Research and Systems Medicine, “Victor Babeș” University of Medicine and Pharmacy, Timisoara, Romania

    Adrian Sturza, Ioana Pavel, Sebastian Ancușa, Corina Danciu, Cristina Dehelean, Oana Duicu & Danina Muntean

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  1. Adrian Sturza
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  2. Ioana Pavel
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Correspondence to Oana Duicu.

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Sturza, A., Pavel, I., Ancușa, S. et al. Quercetin exerts an inhibitory effect on cellular bioenergetics of the B164A5 murine melanoma cell line. Mol Cell Biochem 447, 103–109 (2018). https://doi.org/10.1007/s11010-018-3296-x

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  • DOI: https://doi.org/10.1007/s11010-018-3296-x

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