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Altered extracellular ATP, ADP, and AMP hydrolysis in blood serum of sedentary individuals after an acute, aerobic, moderate exercise session

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Abstract

Nucleotidases participate in the regulation of physiological and pathological events, such as inflammation and coagulation. Exercise promotes distinct adaptations, and can influence purinergic signaling. In the present study, we investigated soluble nucleotidase activities in the blood serum of sedentary young male adults at pre- and post-acute moderate aerobic exercise. In addition, we evaluated how this kind of exercise could influence adenine nucleotide concentrations in the blood serum. Sedentary individuals were submitted to moderate aerobic exercise on a treadmill; blood samples were collected pre- and post-exercise, and serum was separated for analysis. Results showed increases in ATP, ADP, and AMP hydrolysis post-exercise, compared to pre-exercise values. The ecto-nucleotide pyrophosphatase/phosphodiesterase was also evaluated, showing an increased activity post-exercise, compared to pre-exercise. Purine levels were analyzed by HPLC in the blood serum, pre- and post-exercise. Decreased levels of ATP and ADP were found post-exercise, in contrast with pre-exercise values. Conversely, post-exercise levels of adenosine and inosine increased compared to pre-exercise levels. Our results indicate an influence of acute exercise on ATP metabolism, modifying enzymatic behavior to promote a protective biological environment.

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Acknowledgements

This work was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). C.E.J Moritz and L. Rockenbach were recipients of CAPES fellowship.

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Authors and Affiliations

  1. Programa de Pós-Graduação em Medicina: Ciências Médicas, Faculdade de Medicina, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil

    Cesar Eduardo Jacintho Moritz & Ana Maria Oliveira Battastini

  2. Programa de Pós-Graduação em Ciência do Movimento Humano, Escola de Educação Física, Universidade Federado do Rio Grande do Sul, Porto Alegre, RS, Brazil

    Bruno Costa Teixeira & Alvaro Reischak-Oliveira

  3. Progama de Pós-Graduação em Ciências Biológicas: Bioquímica, Instituto de Ciências Básicas, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil

    Liliana Rockenbach & Ana Maria Oliveira Battastini

  4. Departamento de Bioquímica, Instituto de Ciências Básicas, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600 - Anexo, Porto Alegre, RS, 90035-003, Brazil

    Emerson André Casali & Ana Maria Oliveira Battastini

  5. Departamento de Ciências Morfológicas, Instituto de Ciências Básicas, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil

    Emerson André Casali

  6. Departamento de Educação Física, Universidade Regional Integrada do Alto Uruguai e das Missões, São Luiz Gonzaga, RS, Brazil

    Bruno Costa Teixeira

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  1. Cesar Eduardo Jacintho Moritz
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  2. Bruno Costa Teixeira
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  3. Liliana Rockenbach
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Correspondence to Ana Maria Oliveira Battastini.

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Moritz, C.E.J., Teixeira, B.C., Rockenbach, L. et al. Altered extracellular ATP, ADP, and AMP hydrolysis in blood serum of sedentary individuals after an acute, aerobic, moderate exercise session. Mol Cell Biochem 426, 55–63 (2017). https://doi.org/10.1007/s11010-016-2880-1

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