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Effect of vasoactive peptides in Tetrahymena: chemotactic activities of adrenomedullin, proadrenomedullin N-terminal 20 peptide (PAMP) and calcitonin gene-related peptide (CGRP)

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Abstract

Adrenomedullin (AMD), proadrenomedullin N-terminal 20 peptide (PAMP) and calcitonin gene-related peptide (CGRP) were studied for chemotaxis, chemotactic selection and G-actin/F-actin transition in Tetrahymena. The aim of the experiments was to study the effects of two different peptides encoded by the same gene compared to a peptide related to one of the two, but encoded by a different gene, at a low level of phylogeny. The positive, chemotactic effect of ADM and the strong negative, chemorepellent effect of PAMP suggest that in Tetrahymena, the two peptides elicit their chemotactic effects via different signalling mechanisms. The complexity of swimming behaviour modulated by the three peptides underlines that chemotaxis, chemokinesis and some characteristics of migratory behaviour (velocity, tortuosity) are working as a sub-population level complex functional unit. Chemotactic responsiveness to ADM and CGRP is short-term, in contrast to PAMP, which as a chemorepellent ligand, has the ability to select sub-populations with negative chemotactic responsiveness. The different effects of ADM and PAMP on the polymerization of actin networks show that the microtubular structure of cilia is more essential to chemotactic response than are transitions of the actin network. The results draw attention to the characteristic effects of vasoactive peptides at this low level of phylogeny.

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Acknowledgments

This study was funded by a research grant from the Hungarian Scientific Research Fund – OTKA (T17773).

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    Authors and Affiliations

    1. Department of Genetics, Cell and Immunobiology, Semmelweis University, Nagyvárad tér 4., Budapest, 1089, Hungary

      László Kőhidai, Katalin Tóth, Paul Samotik, Kiran Ranganathan & Orsolya Láng

    2. Department of Health Sciences and Sport Medicine, University of Health Science, Alkotás street 44., Budapest, 1123, Hungary

      Miklós Tóth

    3. Division of Pharmacology and Pharmcotherapy, University of Helsinki, P.O.Box 56, 00014, Helsinki, Finland

      Heikki Ruskoaho

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    1. László Kőhidai
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    Correspondence to László Kőhidai.

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    László Kőhidai and Katalin Tóth have contributed equally to this work.

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    Kőhidai, L., Tóth, K., Samotik, P. et al. Effect of vasoactive peptides in Tetrahymena: chemotactic activities of adrenomedullin, proadrenomedullin N-terminal 20 peptide (PAMP) and calcitonin gene-related peptide (CGRP). Mol Cell Biochem 411, 271–280 (2016). https://doi.org/10.1007/s11010-015-2589-6

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