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Fibronectin–integrin mediated signaling in human cervical cancer cells (SiHa)

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Abstract

Interaction between cell surface integrin receptors and extracellular matrix (ECM) components plays an important role in cell survival, proliferation, and migration, including tumor development and invasion of tumor cells. Matrix metalloproteinases (MMPs) are a family of metalloproteinases capable of digesting ECM components and are important molecules for cell migration. Binding of ECM to integrins initiates cascades of cell signaling events modulating expression and activity of different MMPs. The aim of this study is to investigate fibronectin–integrin-mediated signaling and modulation of MMPs. Our findings indicated that culture of human cervical cancer cell (SiHa) on fibronectin-coated surface perhaps sends signals via fibronectin–integrin-mediated signaling pathways recruiting focal adhesion kinase (FAK) extracellular signal regulated kinase (ERK), phosphatidyl inositol 3 kinase (PI-3K), integrin-linked kinase (ILK), nuclear factor-kappa B (NF-κB), and modulates expression and activation of mainly pro-MMP-9, and moderately pro-MMP-2 in serum-free culture medium.

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Acknowledgments

We acknowledge the financial support and inspiration received from Dr. Jaydip Biswas, Director, CNCI, and the financial support from Department of Science and Technology, New Delhi (Grant No. SR/SO/HS-59/2004).

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Authors and Affiliations

  1. Department of Receptor Biology and Tumor Metastasis, Chittaranjan National Cancer Institute, 37, S. P. Mukherjee Road, Kolkata, 700026, West Bengal, India

    Gargi Maity, Shabana Fahreen, Aniruddha Banerji, Paromita Roy Choudhury, Triparna Sen, Anindita Dutta & Amitava Chatterjee

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  1. Gargi Maity
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Correspondence to Amitava Chatterjee.

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Maity, G., Fahreen, S., Banerji, A. et al. Fibronectin–integrin mediated signaling in human cervical cancer cells (SiHa). Mol Cell Biochem 336, 65–74 (2010). https://doi.org/10.1007/s11010-009-0256-5

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  • DOI: https://doi.org/10.1007/s11010-009-0256-5

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