Abstract
Antimicrobial peptides are the first defense molecules against various organisms such as bacteria, fungi and are produced by different organisms. In this study, we aimed to investigate the anticancer effects of an insect antimicrobial peptide, cryptonin isolated from the hemolymph of cicada, Cryptotympana dubia on malignant melanoma cells. Cryptonin decreased the cell viability in a dose and time-dependent manner and these effects are more in metastatic cells than in other cell lines. The release of LDH from damaged cells and the detection of HMGB1 in cell culture media after peptide treatment indicated that cryptonin could induce cell necrosis and this cell death may activate an immune response. The peptide also increased the necrotic cell nucleus stained with ethidium bromide. According to reactive oxygen species production and mitochondrial membrane depolarization results, the apoptotic effect of the peptide was low in melanoma cells. Although the peptide killed the keratinocytes in vitro, cryptonin or cryptonin- derived peptide may have potential use in the treatment of malignant melanoma.
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The data that support the findings of this study are available from the corresponding author upon reasonable request.
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Acknowledgements
G. Torkay and T. Çan would like to acknowledge the financial supports from the Scientific and Technological Research Council of Turkey (TUBITAK) 2210/A General Domestic Graduate Scholarship Program (App No:1649B022101483 and 1649B022113419).
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SS designed the main idea and experiments, did the experiments, evaluated the experimental results statistically, wrote the main manuscript text, found the foundation and GT and TC contributed to the experiments. All authors reviewed the manuscript.
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Sancar, S., Torkay, G. & Çan, T. A Cicada Antimicrobial Peptide, Cryptonin, Exhibits Potent Antitumor Activity by Necrosis on Human Melanoma Cells. Int J Pept Res Ther 29, 68 (2023). https://doi.org/10.1007/s10989-023-10540-3
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DOI: https://doi.org/10.1007/s10989-023-10540-3