Abstract
Late exercise preconditioning (LEP) is known to have a cardioprotection against myocardial injury induced by high-intensity exercise (HE). B-type natriuretic peptide (BNP) carries out the majority of its effects through interaction with natriuretic peptide receptor A (NPR-A) and natriuretic peptide receptor C (NPR-C) to protect heart health. This study aims to investigate the changes in the expression of BNP, NPR-A and NPR-C, during LEP-induced cardioprotection. Male Sprague–Dawley rats (n = 100) were randomly divided into 4 experimental groups (25/group). Rats of LEP model were subjected to an intermittent exercise consisting of 4 periods, each period including 10 min of running at 30 m/min (approximately 75% VO2max) followed by 10 min of intermittent rest. A model of HE-induced myocardial injury was developed by subjecting rats to one bout of high-intensity treadmill exercise at 35 m/min (approximately 80% VO2max) and 0% grade. Following LEP, the levels of BNP and NPR-A were significantly increased, whereas the protein levels of NPR-C were tended to be lower. A group of rats were subjected to LEP followed by HE, and the levels of BNP and NPR-A were significantly elevated in this group, whereas there was a significant decrease in the protein levels of NPR-C. LEP may increase the synthesis and secretion of BNP to assist the cardioprotection via up-regulating of NPR-A and down-regulating of NPR-C.
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Data Availability
The data used to support the findings of this study are available from the corresponding author upon request.
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Not applicable.
Abbreviations
- BNP:
-
B-type natriuretic peptide
- NPR-A:
-
Natriuretic peptide receptor A
- NPR-C:
-
Natriuretic peptide receptor C
- LEP:
-
Late exercise preconditioning
- cGMP:
-
Cyclic guanosine monophosphate
- EP:
-
Exercise preconditioning
- cTnI:
-
Cardiac troponin I
- GAPDH:
-
Glyceraldehydes-3-phosphate dehydrogenase
- IOD:
-
Integral optical density
- I/R:
-
Ischemia/reperfusion
- NPRs:
-
Natriuretic peptide receptors
- NP:
-
Natriuretic peptide
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This study was funded by National Natural Science Foundation of China (Nos. 31071031 and 31471136).
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SSP received the National Natural Science Foundation of China, performed the research design and the manuscript writing. DFW, ZH and YH conducted experiments, performed the data analysis and the manuscript writing. All authors have read and approved the final manuscript.
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All animal experiments were performed by Laboratory animal-Guideline for ethical review of animal welfare (GB/T 35892-2018, China) and approved by Ethics Committee of Science Research in Shanghai University of Sport, China.
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Wan, DF., Hao, Z., Huang, Y. et al. Late Exercise Preconditioning Regulates BNP Increasing to Assist the Cardioprotection via Up-Regulation of NPR-A and Down-Regulation of NPR-C in Rat Myocardium. Int J Pept Res Ther 28, 13 (2022). https://doi.org/10.1007/s10989-021-10323-8
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DOI: https://doi.org/10.1007/s10989-021-10323-8