Abstract
Antimicrobial peptides (AMPs) are considered ideal antibiotic candidates, but their therapeutic ability and application are restricted by their limited formulation. Nanoliposome encapsulation can lower the haemolytic toxicity of drugs. CGA-N9, a peptide derived from chromogranin A, is an AMP with high antifungal activity against Candida tropicalis. Herein, CGA-N9-encapsulated nanoliposomes were prepared and characterized, and their antagonistic activity against C. tropicalis and haemolytic activity were studied. The results showed that the CGA-N9 nanoliposomes had a particle size of 127.5 ± 12.8 nm and an encapsulation efficiency of 87.9%, as determined by the high-performance liquid chromatography (HPLC) method established herein. The minimum inhibitory concentration (MIC) of the CGA-N9 nanoliposomes was 7.8 μg/mL, which was higher than that of free CGA-N9 (3.9 μg/mL), and no haemolysis was detected at the maximum concentration tested (250 μg/mL). These results provide an experimental reference for the practical use of peptides in the clinic.
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Funding
This study was funded by the National Natural Science Foundation of China (31572264, 31071922); the Innovative Research Team (in Science and Technology) at the University of Henan Province (19IRTSTHN008); the Innovative Funds Plan of Henan University of Technology (2020ZKCJ23); the Science and Technology Department of Henan Province (212102311045, 212102310322).
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RL and BZ proposed the study. LH, XW and LZ performed the experiments. RL and BZ performed the statistical analysis. RL drafted the manuscript.
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Liang Huang declares that he has no conflicts of interest. Ruifang Li declares that she has no conflicts of interest. Xueqin Wang declares that she has no conflict of interest. Lan Zhang declares that she has no conflicts of interest. Beibei Zhang declares that she has no conflicts of interest.
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Huang, L., Li, R., Wang, X. et al. Preparation and Characterization of Nanoliposomes Loaded with the Antimicrobial Peptide CGA-N9. Int J Pept Res Ther 27, 2727–2734 (2021). https://doi.org/10.1007/s10989-021-10286-w
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DOI: https://doi.org/10.1007/s10989-021-10286-w