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Antimicrobial Substance Produced by Pseudomonas aeruginosa Isolated from Slaughterhouse Sediment: Physicochemical Characterization, Purification, and Identification

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Abstract

This study aimed to produce the novel bacteriocin from Pseudomonas aeruginosa 43. The bacteriocin was purified through 80% ammonium sulfate precipitation, cation exchange chromatography, and gel filtration, and the specific antimicrobial activity was 400 AU/ml of protein. The molecular weight of purified bacteriocin was 10 kDa determined by SDS-PAGE. It was shown to be heat stable at 121 °C for 30 min and functioned well at low pH in a range of 3–7. Reduction of activity was shown after treatment with proteinase K, trypsin, papain, and α-amylase that confirmed its proteinaceous nature. Bacteriocin also showed its stability against various organic solvents and chemical reagents. Scanning electron microscope analysis indicated that bacteriocin from P. aeruginosa 43 damaged the morphology of methicillin-resistant Staphylococcus aureus (MRSA). Also, the novel bacteriocin exhibited an enhanced ability to impair biofilm formation and to reduce the density of established biofilms. LC–MS analysis study showed elastase is a novel peptide produced by P. aeruginosa 43 which has broad spectrum of inhibition against MRSA. The study suggested that bacteriocin from P. aeruginosa 43 could be developed as a antimicrobial agent for skin infection.

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Acknowledgements

The authors gratefully acknowledge the Department of Science and Technology, New Delhi for providing financial supports under the DST-WOS-A start of Grant for (DST/SR/WOS- A/LS-629/2012(G)), Scheme. Also the Department of Biological Science and Technology, National Pingtung University of Science and Technology, Taiwan for providing financial supports and lab specialty.

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Authors and Affiliations

  1. Regenerative Medicine Laboratory, Department of Zoology, School of Life Sciences, Periyar University, Salem, Tamil Nadu, 636011, India

    Dhanam Selvam, Arumugam Thangarasu & Kayalvizhi Nagarajan

  2. Functional Genomics Lab, Department of Biological Science and Technology, National Pingtung University of Science and Technology, Neipu, Pingtung, 91201, Taiwan

    Douglas J. H. Shyu

  3. School of Life Sciences, Central University of Tamil Nadu, Thiruvarur, Tamil Nadu, 610005, India

    Rameshkumar Neelamegam

  4. Madurai Kamaraj University, Madurai, Tamil Nadu, 625 021, India

    Krishnan Muthukalingan

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  1. Dhanam Selvam
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  2. Arumugam Thangarasu
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  3. Douglas J. H. Shyu
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  4. Rameshkumar Neelamegam
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  5. Krishnan Muthukalingan
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  6. Kayalvizhi Nagarajan
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Correspondence to Kayalvizhi Nagarajan.

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Selvam, D., Thangarasu, A., Shyu, D.J.H. et al. Antimicrobial Substance Produced by Pseudomonas aeruginosa Isolated from Slaughterhouse Sediment: Physicochemical Characterization, Purification, and Identification. Int J Pept Res Ther 27, 887–897 (2021). https://doi.org/10.1007/s10989-020-10135-2

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  • DOI: https://doi.org/10.1007/s10989-020-10135-2

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