Abstract
The skin secretions of amphibians are a rich source of bioactive peptides. We isolated chensirin-1 and chensirin-2 from the skin secretion of the Chinese frog Rana chensinensis. Sephadex-G-50 and RP-HPLC were employed to purify these peptides. The amino acid sequences of these peptides were VLPLVGNLLNDLLGE and IIPLPLGYFAKKT, respectively, as determined by Edman degradation. The molecular weights were 1578.7 and 1460.8 Da, respectively, as analyzed by HPLC-ESI-MS. The chensirin cDNA was cloned by 5′ and 3′ amplification of cDNA ends, synthesized and purified. The antibacterial activities of the chensirins were tested using minimum inhibitory concentration, the results indicated that chensirins inhibit the growth of gram-negative and gram-positive bacteria. Among them, chensirin-1 is a novel peptide with a higher antibacterial activity compared to other similar antimicrobial peptides. These low molecular weight peptides with good antimicrobial efficacy are considered potential sources for developing new antimicrobial agents to improve traditional drug resistance.
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Acknowledgements
We thank the Ministry of Science and Technology of the People’s Republic of China [2013BAD16B09-03] and Jilin Provincial Science and Technology Department [20180201053NY] for financial support.
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Manyu Wu, Yang He, Djerry Yvan Arold Dinghani, Yuhua Wang, Yaohui Hu, Huan Wang, Bixiang Wang, Bo Lv, Hansong Yu and Liankui Wen declared that they have no conflict of interest.
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Wu, M., He, Y., Dinghani, D.Y.A. et al. Purification and cDNA Cloning of Antimicrobial Peptides from the Skin Secretion of the Chinese Frog Rana chensinensis. Int J Pept Res Ther 27, 293–300 (2021). https://doi.org/10.1007/s10989-020-10074-y
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DOI: https://doi.org/10.1007/s10989-020-10074-y