Abstract
Antioxidant enzymes protect cells against reactive oxygen species. The level of antioxidant enzymes is reduced almost in cancer cells. The effect of brevinine-2R (B2R) peptide on antioxidant enzyme activities in A549 cell line were investigated and results compared with glutathione (GSH). Catalase, superoxide dismutase (SOD) and glutathione peroxidase of A549 extract cells had their most activities in treated cells at 4.8 µM of B2R and 9.5 µM of GSH for 24 h. Cell attachment was also increased in the presence of B2R and GSH peptide. Thiol status, antioxidant capacity and reactive oxygen species (ROS) of the treated A549 cells were measured. B2R showed its most ROS level, antioxidant capacity and thiol status in the treated cells at 4.8 µM of peptide. B2R peptide like GSH has cytoprotective effect on A549 cells by strengthening the cellular antioxidant system thereby confirming potential pharmaceutical property of the peptide.
Similar content being viewed by others
References
Alalwani SM, Sierigk J, Herr C, Pinkenburg O, Gallo R, Vogelmeier C, Bals R (2010) The antimicrobial peptide LL-37 modulates the inflammatory and host defense response of human neutrophils. Eur J Immunol 40:1118–1126. https://doi.org/10.1002/eji.200939275
Almoazen H, Bhattacharjee H, Samsa AC, Pate S (2010) Stability of mesna in ReadyMed infusion devices. Ann Pharmacother https://doi.org/10.1345/aph.1M390
Beers RF, Sizer IW (1952) A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem 195:133–140
Björkman U, Ekholm R (1995) Hydrogen peroxide degradation and glutathione peroxidase activity in cultures of thyroid cells. Mol Cell Endocrinol 111:99–107. https://doi.org/10.1016/0303-7207(95)03552-I
Chelikani P, Fita I, Loewen PC (2004) Diversity of structures and properties among catalases. Cell Mol Life Sci. https://doi.org/10.1007/s00018-003-3206-5
Cho J, Lee DG (2011) The antimicrobial peptide arenicin-1 promotes generation of reactive oxygen species and induction of apoptosis. Biochim Biophys Acta 1810:1246–1251. https://doi.org/10.1016/j.bbagen.2011.08.011
Dafre AL, Medeiros ID, Müller IC, Ventura EC, A.B (2004) Novel antioxidant enzymes and thiol/disulfide status in the digestive gland of the brown mussel Perna perna exposed to lead and paraquat peptides derived from enzymatic hydrolysates of Macadamia protein. Chem Biol Interact 149:97–105. https://doi.org/10.1016/j.cbi.2004.07.002
Eliassen LT, Berge G, Leknessund A, Wikman M, Lindin I, Løkke C, Ponthan F, Johnsen JI, Sveinbjørnsson B, Kogner P, Flægstad T, Rekdal Ø (2006) The antimicrobial peptide, Lactoferricin B, is cytotoxic to neuroblastoma cells in vitro and inhibits xenograft growth in vivo. Int J Cancer 119:493–500. https://doi.org/10.1002/ijc.21886
Eruslanov E, Kusmartsev S (2010) Identification of ROS using oxidized DCFDA and flow-cytometry. Methods Mol Biol 594:57–72. https://doi.org/10.1007/978-1-60761-411-1_4
Gaspar D, Salomé Veiga A, Castanho MARB (2013) From antimicrobial to anticancer peptides. A review. Front Microbiol. https://doi.org/10.3389/fmicb.2013.00294
Ghavami S, Asoodeh A, Klonisch T, Halayko AJ, Kadkhoda K, Kroczak TJ, Gibson SB, Booy EP, Naderi-Manesh H, Los M (2008) Brevinin-2R1 semi-selectively kills cancer cells by a distinct mechanism, which involves the lysosomal-mitochondrial death pathway. J Cell Mol Med 12:1005–1022. https://doi.org/10.1111/j.1582-4934.2008.00129.x
Han Z, Zhang W, Luo W, Li J (2016) Novel antioxidant peptides derived from enzymatic hydrolysates of macadamia protein. J Biosci Med 4:63428. https://doi.org/10.4236/jbm.2016.42002
Hayyan M, Hashim MA, Alnashef IM (2016) Superoxide ion: generation and chemical implications. Chem Rev 116:3029–3085. https://doi.org/10.1021/acs.chemrev.5b00407
Homayouni-Tabrizi M, Asoodeh A, Mashreghi M, Rezazade Bazaz M, Oskuee KR, Darroudi M (2016) Attachment of a frog skin-derived peptide to functionalized cerium oxide nanoparticles. Int J Pept Res Ther 22:505–510. https://doi.org/10.1007/s10989-016-9531-y
Huang D, Boxin OU, Prior RL (2005) The chemistry behind antioxidant capacity assays. J Agric Food Chem https://doi.org/10.1021/jf030723c
Huang T, Li J, Zhang C, Hong Q, Jiang D, Ye M, Duan S (2016) Distinguishing lung adenocarcinoma from lung squamous cell carcinoma by two hypomethylated and three hypermethylated genes: a meta-analysis. PLoS ONE. https://doi.org/10.1371/journal.pone.0149088
Liou M-Y, Storz P (2010) Reactive oxygen species in cancer. Free Radic Res. https://doi.org/10.3109/10715761003667554.Reactive
Lu Y, Zhang T-F, Shi Y, Zhou H-W, Chen Q, Wei B-Y, Wang X, Yang T-X, Chinn YE, Kang J, Fu C-Y (2016) PFR peptide, one of the antimicrobial peptides identified from the derivatives of lactoferrin, induces necrosis in leukemia cells. Sci Rep 6:20823. https://doi.org/10.1038/srep20823
Maurya DK, Nandakumar N, Devasagayam TPA (2011) Anticancer property of gallic acid in A549, a human lung adenocarcinoma cell line, and possible mechanisms. J Clin Biochem Nutr 48:85–90. https://doi.org/10.3164/jcbn.11-004FR
Mehrnejad F, Naderi-Manesh H, Ranjbar B, Maroufi B, Asoodeh A, Doustdar F (2008) PCR-based gene synthesis, molecular cloning, high level expression, purification, and characterization of novel antimicrobial peptide, Brevinin-2R, in Escherichia coli. Appl Biochem Biotechnol 149:109–118. https://doi.org/10.1007/s12010-007-8024-z
Melik-Adamyan W, Bravo J, Carpena X, Switala J, Maté MJ, Fita I, Loewen PC (2001) Substrate flow in catalases deduced from the crystal structures of active site variants of HPII from Escherichia coli. Proteins Struct Funct Genet 44:270–281. https://doi.org/10.1002/prot.1092
Moreno I, Pichardo S, Jos A, Gómez-Amores L, Mate A, Vazquez CM, Cameán AM (2005) Antioxidant enzyme activity and lipid peroxidation in liver and kidney of rats exposed to microcystin-LR administered intraperitoneally. Toxicon 45:395–402. https://doi.org/10.1016/j.toxicon.2004.11.001
Oberley TD, Oberley LW (1997) Antioxidant enzyme levels in cancer. Histol Histopathol 12:525–535
Pascariu M, Nevoie A, Constantinescu D, Carasevici E, Luchian T (2011) The Evaluation of biological effect of cytotoxic peptides on tumor cell lines. Dig J Nanomater Biostruct 7:79–84. https://doi.org/10.1016/j.bbamem.2007.11.008
Reliene R, Schiestl RH (2006) Antioxidant N-acetyl cysteine reduces incidence and multiplicity of lymphoma in Atm deficient mice. DNA Repair 5:852–859. https://doi.org/10.1016/j.dnarep.2006.05.003
Sohal RS, Mockett RJ, Orr WC (2002) Mechanisms of aging: an appraisal of the oxidative stress hypothesis. Free Radic Biol Med. https://doi.org/10.1016/S0891-5849(02)00886-9
Stratil P, Klejdus B, Kuban V (2006) Determination of total content of phenolic compounds and their antioxidant activity in vegetables s evaluation of spectrophotometric methods. J Agric Food Chem 54:607–616. https://doi.org/10.1021/jf052334j
Su X, Dong C, Zhang J, Su L, Wang X, Cui H, Chen Z (2014) Combination therapy of anti-cancer bioactive peptide with Cisplatin decreases chemotherapy dosing and toxicity to improve the quality of life in xenograft nude mice bearing human gastric cancer. Cell Biosci 4:7. https://doi.org/10.1186/2045-3701-4-7
Tainer JA, Getzoff ED, Richardson JS, Richardson DC (1983) Structure and mechanism of copper, zinc superoxide dismutase. Nature 306:284–287. https://doi.org/10.1038/306284a0
Tsai PW, Yang CY, Chang HT, Lan CY (2011) Human antimicrobial peptide LL-37 inhibits adhesion of Candida albicans by interacting with yeast cell-wall carbohydrates. PLoS ONE. https://doi.org/10.1371/journal.pone.0017755
Umino T, Wang H, Zhu Y, Liu X, Manouilova LS, Spurzem JR, Leuschen MP, Rennard SI (2000) Modification of type I collagenous gels by alveolar epithelial cells. Am J Respir Cell Mol Biol 22:702–707. https://doi.org/10.1165/ajrcmb.22.6.3806
Weydert CJ, Cullen JJ (2010) Measurement of superoxide dismutase, catalase and glutathione peroxidase in cultured cells and tissue. Nat Protoc 5:51–66. https://doi.org/10.1038/nprot.2009.197
Woolliams JA, Wiener G, Anderson PH, McMurray CH (1983) Variation in the activities of glutathione peroxidase and superoxide dismutase and in the concentration of copper in the blood in various breed crosses of sheep. Res Vet Sci 34:253–256
Zhang P, Gao WY, Turner S, Ducatman BS (2003) Gleevec (STI-571) inhibits lung cancer cell growth (A549) and potentiates the cisplatin effect in vitro. Mol Cancer 2:1. https://doi.org/10.1186/1476-4598-2-1
Acknowledgements
The authors appreciate the support provided by the Ferdowsi University of Mashhad Research and Technology council (Grant Nos.: 3/42984, 1395/12/03).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
Bahareh Ghodsi-Moghadam and Ahmad Asoodeh declare that they have no conflict of interest.
Research Involving Human and Animal Rights
This article does not contain any studies with human or animal subjects performed by any of the authors.
Rights and permissions
About this article
Cite this article
Ghodsi-Moghadam, B., Asoodeh, A. The Impact of Brevinin-2R Peptide on Oxidative Statues and Antioxidant Enzymes in Human Epithelial Cell Line of A549. Int J Pept Res Ther 25, 1065–1074 (2019). https://doi.org/10.1007/s10989-018-9754-1
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10989-018-9754-1