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Buffalo Milk Casein Derived Decapeptide (YQEPVLGPVR) Having Bifunctional Anti-inflammatory and Antioxidative Features Under Cellular Milieu

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Abstract

Antioxidants having anti-inflammatory potential will be useful in reducing the progression of many lifestyle associated diseases. Under present investigation, buffalo casein derived decapeptide (YQEPVLGPVR) displayed anti-inflammatory response by suppressed (p < 0.01) murine splenocytes proliferation, reduced inflammatory cytokine levels (Interferon-γ) besides elevated levels of regulatory cytokines (Interleukin-10 and Transforming Growth Factor-β) in splenocyte culture supernatant. Decapeptide also improved the phagocytosis (p < 0.01) of peritoneal macrophages. Subsequently, antioxidative feature of the peptide was also identified by efficient (p < 0.01) free radical scavenging using chemical assays (ABTS: 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid and ORAC: oxygen radical absorption capacity methods) which was later confirmed for protective action against H2O2 mediated oxidative stress on intestinal epithelial cells by inhibition (p < 0.01) of cellular ROS generation, oxidative products formation along with elevated (p < 0.01) activities of antioxidative enzymes (catalase and glutathione peroxidase). Declined (p < 0.01) mRNA expression of a transcription factor (Nuclear factor erythroid 2-related factor: Nrf-2) involved in redox signalling further established the protective effect of peptide against oxidative stress induced injury.

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Acknowledgements

The authors are grateful to the SERB-DST/MOFPI (Department of Science and Technology) and ICAR-National Dairy Research Institute (NDRI), Karnal for providing funding and laboratory facilities to carry out this piece of work.

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Authors and Affiliations

  1. Animal Biochemistry Division, ICAR-National Dairy Research Institute, Karnal, Haryana, 132001, India

    Kandukuri Sowmya, Mohd Iqbal Bhat, Suman Kapila & Rajeev Kapila

  2. Dairy Chemistry Division, ICAR-National Dairy Research Institute, Karnal, Haryana, 132001, India

    Rajesh Kumar Bajaj

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  1. Kandukuri Sowmya
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  2. Mohd Iqbal Bhat
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Correspondence to Rajeev Kapila.

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Sowmya, K., Bhat, M.I., Bajaj, R.K. et al. Buffalo Milk Casein Derived Decapeptide (YQEPVLGPVR) Having Bifunctional Anti-inflammatory and Antioxidative Features Under Cellular Milieu. Int J Pept Res Ther 25, 623–633 (2019). https://doi.org/10.1007/s10989-018-9708-7

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