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Release of Proteolysis Products with ACE-Inhibitory and Antioxidant Activities in Probiotic Yogurt Containing Different Levels of Fat and Prebiotics

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Abstract

In this study, the effects of prebiotic ingredients (inulin and wheat fiber) and fat percentage (0, 2 and 3.5%) on physicochemical properties, degree of proteolysis, antioxidant and ACE-inhibitory activity within 21 days storage of yogurt containing probiotic Lactobacillus casei at 5 ± 1 °C were evaluated. The results of statistical analysis showed that the application of prebiotics led to a significant increase in water-holding capacity, proteolysis and ACE-inhibitory activity of samples. The degree of proteolysis in yogurt increased as storage time elapsed (P < 0.05) but when proteolysis exceeded a certain threshold, this trend began to decline. Also, during storage time, water holding capacity reduced initially but increased thereafter. Moreover, based on our findings, the survival of L. casei, in samples treated with inulin and wheat fiber, increased significantly as compared to the control sample (P < 0.05) whereas the fat percentage did not reflect significant (P < 0.05) effect on the survival of probiotic bacteria. Furthermore, prebiotic ingredients and the presence of probiotic cultures had significant (P < 0.05) effect on the antioxidant activity of samples.

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References

  • Amirdivani SH, Salihin Baba A (2011) Change in yogurt fermentation characteristic and antioxidant potential and in vivo inhibition of angiotensin-1 converting enzyme upon the inclusion of peppermint, dill and basil. Food Sci Technol 44:1458–1464

    CAS  Google Scholar 

  • Amiri A, Amiri A (2017) Antioxidants and heart disease; current knowledge. Ann Res Antioxid 2(1):e03

    Google Scholar 

  • Ashar MN, Chand R (2004) Fermented milk containing ACE-inhibitory peptides reduces blood pressure in middle aged hypertensive subjects. Milchwissenschaft 59:363–366

    CAS  Google Scholar 

  • Aureli P, Capurso L, Castellazzi AM, Clerici M, Giovannini M, Morelli L, Poli A, Pregliasco F, Salvini F, Zuccotti GV (2011) Probiotics and health: an evidence-based review. Pharmacol Res 63:366–376

    Article  CAS  PubMed  Google Scholar 

  • Behera P, Kumar R, Sandeep IVR, Kapila R, Dang AK, Kapila S (2013) Casein hydrolysates enhance osteoblast proliferation and differentiation in mouse bone marrow culture. Food Biosci 2:24–30

    Article  CAS  Google Scholar 

  • Bertazzoni E, Donelli G, Midtvedt T, Nicoli J, Sanz Y (2013) Probiotics and clinical effects: is the number what counts? J Chemother 25(4):193–212

    Article  PubMed  Google Scholar 

  • Blois MS (1958) Antioxidant determinations by the use of a stable free radical. Nature 26:1199–1200

    Article  Google Scholar 

  • Bron PA, Kleerebezem M, Brummer RJ, Cani PD, Mercenier A, MacDonald TT, Garcia-Ródenas CL, Wells JM (2017) Can probiotics modulate human disease by impacting intestinal barrier function? Br J Nutr 117:93–107

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Burgain J, Gaiani C, Linder M, Scher J (2011) Encapsulation of probiotic living cells: from laboratory scale to industrial applications. J Food Eng 104:467–483

  • Cheng X, Gao DX, Song JJ, Ren FZ, Mao XY (2015) Casein glycomacropeptide hydrolysate exerts cytoprotection against H2O2-induced oxidative stress in RAW 264.7 macrophages via ROS-dependent heme oxygenase-1 expression. RSC Adv 5:4511–4523

    Article  CAS  Google Scholar 

  • Church FC, Swaisgood HE, Porter DH, Catignani GL (1983) Spectrophotometric assay using o-phthaldialdehyde for determination of proteolysis in milk and isolated milk proteins. J Dairy Sci 66:1219–1227

    Article  CAS  Google Scholar 

  • CODEX standard for fermented milks (2010) Milk and milk products (2nd edn). CODEX STAN 243. Adopted in 2003. Revision 2008, 2010

  • Coppey LJ, Davidson EP, Rinehart TW, Gellett JS, Oltman CL, Lund DD, Yorek MA (2006) ACE inhibitor or Angiotensin II receptor antagonist attenuates diabetic neuropathy in Streptozotocin-induced diabetic rats. Diabetes 55:341–348

    Article  CAS  PubMed  Google Scholar 

  • Cumby N, Zhong Y, Naczk M, Shahidi F (2008) Antioxidant activity and water-holding capacity of canola protein hydrolysates. Food Chem 109:144–148

    Article  CAS  PubMed  Google Scholar 

  • Cushman DW, Cheung HS (1971) Spectrophotometric assay and properties of the angiotensin-converting enzyme of rabbit lung. Biochem Pharmacol 20:1637–1648

    Article  CAS  PubMed  Google Scholar 

  • de Morais EC (2016) Prebiotic addition in dairy products: processing and health benefits. In: Watson RR, Preedy VR (eds) Probiotics, prebiotics, and synbiotics—bioactive foods in health promotion. Elsevier, Amsterdam

    Google Scholar 

  • Donkor ON, Henriksson A, Vasiljevic T, Shah NP (2005) Probiotic strains as starter cultures improve angiotensin-converting enzyme inhibitory activity in soy yoghurt. J Food Sci 70:M375–M381

    Article  CAS  Google Scholar 

  • Donkor ON, Nilmini SLI, Stolic P, Vasiljevic T, Shah NP (2006) Survival and activity of selected probiotic organisms in set-type yoghurt during cold storage. Int Dairy J 17:657–665

    Article  CAS  Google Scholar 

  • Donkor ON, Henriksson A, Singh TK, Vasilijevic T, Shah NP (2007a) ACE-inhibitory activity of probiotic yoghurt. Int Dairy J 17:1321–1331

    Article  CAS  Google Scholar 

  • Donkor ON, Henriksson A, Vasiljevic T, Shah NP (2007b) α-Galactosidase and proteolytic activities of selected probiotic and dairy cultures in fermented soymilk. Food Chem 104(1):10–20

    Article  CAS  Google Scholar 

  • Donkor ON, Henriksson A, Vasiljevic T, Shah NP (2007c) Proteolytic activity of dairy lactic acid bacteria and probiotics as determinant of growth and in vitro angiotensin-converting enzyme inhibitory activity in fermented milk. Le Lait. INRA Ed 87(1):21–38

    CAS  Google Scholar 

  • EFSA (2010) Scientific Opinion on the substantiation of health claims related to live yoghurt cultures and improved lactose digestion (ID 1143, 2976) pursuant to Article 13(1) of Regulation (EC) No 1924/2006. EFSA J 8(10):1763

    Google Scholar 

  • Elmadfa I, Meyer A (2008) Body composition, changing physiological functions and nutrient requirements of the elderly. Ann Nutr Metab 52:2–5

    Article  CAS  PubMed  Google Scholar 

  • Evivie SE, Huo GC, Igene JO, Bian X (2017) Some current applications, limitations and future perspectives of lactic acid bacteria as probiotics. Food Nutr Res 61:1–16

    Article  CAS  Google Scholar 

  • FAO/WHO (2002) Report of a joint FAO/WHO working group report on drafting guidelines for the evaluation of probiotics in food. FAO/WHO, London

    Google Scholar 

  • Farnworth ER, Champagne CP (2016) Production of probiotic cultures and their incorporation into foods. In: Watson RR, Preedy VR (eds) Probiotics, prebiotics, and synbiotics—bioactive foods in health promotion. Elsevier, Amsterdam

    Google Scholar 

  • Fekete AA, Givens DI, Lovegrove JA (2015) Casein-derived lactotripeptides reduce systolic and diastolic blood pressure in a meta-analysis of randomized clinical trials. Nutrients 7:659–681

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • FitzGerald RJ, Murray BA, Walsh DJ (2004) Hypotensive peptides from milk proteins. J Nutr 134:980S–988S

    Article  CAS  PubMed  Google Scholar 

  • Flattery MP, Sica DA (2007) Angiotensin-converting enzyme inhibitor–related angioedema: recognition and treatment. Prog Cardiovasc Nurs 22:47–51

    Article  PubMed  Google Scholar 

  • Franklin SS, Wong ND (2013) Hypertension and cardiovascular disease: contributions of the framingham heart study. Global Heart 8(1):49–57

    Article  PubMed  Google Scholar 

  • Gallego CG, Salminen S (2016) Novel probiotics and prebiotics: how can they help in human gut microbiota dysbiosis? Appl Food Biotechnol 3(2):72–81

    CAS  Google Scholar 

  • Gamon LF, Wille U (2016) Oxidative damage of biomolecules by the environmental pollutants NO2 • and NO3 •. Acc Chem Res 49:2136–2145

    Article  CAS  PubMed  Google Scholar 

  • Germani A, Luneia R, Nigro F, Vitiello V, Donini LM, del Balzo V (2014) The yogurt amino acid profile’s variation during the shelf-life. Annali di Igiene 26(3):205–212

    CAS  PubMed  Google Scholar 

  • Gobbetti M, Minervini F, Rizzello CG (2004) Angiotensin I-converting-enzyme-inhibitory and antimicrobial bioactive peptides. Int J Dairy Technol 57:172–188

    Article  Google Scholar 

  • Gonzalez-Gonzalez CR, Tuohy KM, Jauregi P (2011) Production of angiotensin-I-converting enzyme (ACE) inhibitory activity in milk fermented with probiotic strains: effects of calcium, pH and peptides on the ACE-inhibitory activity. Int Dairy J 21:615–622

    Article  CAS  Google Scholar 

  • Hata Y, Yamamoto M, Ohni M, Nakajima K, Nakamura Y, Takano T (1996) A placebo-controlled study of the effect of sour milk on blood pressure in hypertensive subjects. Am J Clin Nutr 64:767–771

    Article  CAS  PubMed  Google Scholar 

  • Hati S, Patel N, Sakure A, Mandal S (2017) Influence of whey protein concentrate on the production of antibacterial peptides derived from fermented milk by acid bacteria. Int J Pept Res Ther. https://doi.org/10.1007/s10989-017-9596-2

    Article  Google Scholar 

  • Hsieh CC, Hernández-Ledesma B, Fernández-Tomé S, Weinborn V, Barile D, de Moura Bell JMLN. (2015) Milk proteins, peptides, and oligosaccharides: effects against the 21st century disorders. Biomed Res Int 2015:1–16

    Google Scholar 

  • Kumar M, Behare PV, Mohania D, Arora S, Kaur A, Nagpal R (2009) Health-promoting probiotic functional foods: potential and prospects. Agro Food Ind Hi Tech 20(3):29–33

    Google Scholar 

  • Kumar M, Kumar A, Nagpal R, Mohania D, Behare P, Verma V, Kumar P, Poddar D, Aggarwal PK, Henry CJK, Jain S, Yadav H (2010) Cancer-preventing attributes of probiotics: an update. Int J Food Sci Nutr 61(5):473–496

    Article  CAS  PubMed  Google Scholar 

  • Kumar M, Nagpal R, Hemalatha R, Yadav H, Marotta F (2016) Probiotics and prebiotics for promoting health: through gut microbiota. In: Watson RR, Preedy VR (eds) Probiotics, prebiotics, and synbiotics—bioactive foods in health promotion. Elsevier, Amsterdam

    Google Scholar 

  • Kunda PB, Benavente F, Catala-Clariana S, Gimenez E, Barbosa J, Sanz-Nebot V (2012) Identification of bioactive peptides in a functional yogurt by micro liquid chromatography time-of-flight mass spectrometry assisted by retention time prediction. J Chromatogr A 1229:121–128

    Article  CAS  PubMed  Google Scholar 

  • Kunji ERS, Mierau I, Hagting A, Poolman B, Konings WN (1996) The proteolytic systems of lactic acid bacteria. Antonie Leeuwenhoek 70:187–221

    Article  CAS  PubMed  Google Scholar 

  • Leclerc PL, Gauthier SF, Bachelard H, Santure M, Roy D (2002) Antihypertensive activity of casein-enriched milk fermented by Lactobacillus helveticus. Int Dairy J 12:995–1004

    Article  CAS  Google Scholar 

  • Li-Chan ECY (2015) Bioactive peptides and protein hydrolysates: research trends and challenges for application as nutraceuticals and functional food ingredients. Curr Opin Food Sci 1:28–37

    Article  Google Scholar 

  • Liu M, Bayjanov JR, Renckens B, Nauta A, Siezen RJ (2010) The proteolytic system of lactic acid bacteria revisited: a genomic comparison. BMC Genom 11:36

    Article  CAS  Google Scholar 

  • Lourens-Hattingh A, Viljoen CB (2001) Yogurt as probiotic carrier food. Int Dairy J 11:1–17

    Article  Google Scholar 

  • Mani-López E, Palou E, López-malo A (2014) Probiotic viability and storage stability of yogurts and fermented milks prepared with several mixtures of lactic acid bacteria. J Dairy Sci 97:2578–2590

    Article  CAS  PubMed  Google Scholar 

  • Mann B, Athira S, Sharma R, Bajaj R (2017) Bioactive peptides in yogurt. In: Shah NP (ed), Yogurt in health and disease prevention. Elsevier, Amsterdam, pp 411–426

    Chapter  Google Scholar 

  • Markowiak P, Śliżewska K (2017) Effects of probiotics, prebiotics, and synbiotics on human health. Nutrients 9(1021):1–30

    Google Scholar 

  • Martin A (2001) Functional foods: from marketing to public health. OCL-Oleagineux Corps Gras Lipides 8(4):310–311

    Article  Google Scholar 

  • Matsuzaki T (2003) Health properties of fermented milk with Lactobacillus casei strain shirota (LcS). In: Farnworth ER (ed) Handbook of fermented functional foods, 2nd edn. CRC Press, Boca Raton, pp 145–175

    Chapter  Google Scholar 

  • Meisel H, FitzGerald RJ (2003) Biofunctional peptides from milk proteins: mineral binding and cytomodulatory effects. Curr Pharm Des 9:1289–1295

    Article  CAS  PubMed  Google Scholar 

  • Minelli EB, Benini A, Marzotto M, Sbarbati A, Ruzzenente O, Ferrario R, Hendriks H, Dellaglio F (2004) Assessment of novel probiotic Lactobacillus casei strains for the production of functional dairy foods. Int Diary J 14(8):723–736

    Article  CAS  Google Scholar 

  • Mohania D, Kansal VK, Shah D, Nagpal R, Kumar M, Gautam SK, Singh B, Behare PV (2013) Therapeutic effect of probiotic Dahi on plasma, aortic, and hepatic lipid profile of hypercholesterolemic rats. J Cardiovasc Pharmacol Therap 18(5):490–497

    Article  CAS  Google Scholar 

  • Murray BA, FitzGerald RJ (2007) Angiotensin converting enzyme inhibitory peptides derived from food proteins: biochemistry, bioactivity and production. Curr Pharm Des 13:773–791

    Article  CAS  PubMed  Google Scholar 

  • Niamah AK, Al-Sahlany STG, Al-Manhel AJ (2016) Gum arabic uses as prebiotic in yogurt production and study effects on physical,chemical properties and survivability of probiotic bacteria during cold storage. World Appl Sci J 34(9):1190–1196

    CAS  Google Scholar 

  • Nielsen PM, Petersen D, Dambmann C (2001) Improved method for determining food protein degree of hydrolysis. Food Chem Toxicol 5:642–646

    Google Scholar 

  • Nielsen MS, Martinussen T, Flambard B, Sørensen KI, Otte J (2009) Peptide profiles and angiotensin-I-converting enzyme inhibitory activity of fermented milk products: effect of bacterial strain, fermentation pH, and storage time. Int Dairy J 19:155–165

    Article  CAS  Google Scholar 

  • Nongonierma AB, FitzGerald RJ (2015) The scientific evidence for the role of milk protein-derived bioactive peptides in humans: a review. J Funct Foods 17:640–656

    Article  CAS  Google Scholar 

  • O’Loughlin IB, Kelly PM, Murray BA, FitzGerald RJ, Brodkorb A (2015) Molecular characterization of whey protein hydrolysate fractions with ferrous chelating and enhanced iron solubility capabilities. J Agric Food Chem 63:2708–2714

    Article  CAS  PubMed  Google Scholar 

  • Okoduwa SIR, Umar IA, Ibrahim S, Bello F, Habila N (2015) Age-dependent alteration of antioxidant defense system in hypertensive and type-2 diabetes patients. J Diabetes Metab Disord 14(32):1–9

    Google Scholar 

  • Pandey KB, Mehdi MM, Maurya PK, Rizvi SI (2010) Plasma protein oxidation and its correlation with antioxidant potential during human aging. Dis Mark 29:31–36

    Article  CAS  Google Scholar 

  • Papadimitriou CG, Vafopoulou-Mastrojiannaki A, Silva SV, Gomes AM, Malcata FX, Ali chanidis E (2007) Identification of peptides in traditional and probiotic sheep milk yoghurt with angiotensin I-converting enzyme (ACE)-inhibitory activity. Food Chem 105:647–656

    Article  CAS  Google Scholar 

  • Park YW, Nam MS (2015) Bioactive peptides in milk and dairy products: a review. Kor J Food Sci Anim Resour 35(6):831–840

    Article  Google Scholar 

  • Parnell-Clunies EM, Kakuda Y, Mullen K, Arnott DR, Deman JM (1986) Physical properties of yogurt—a comparison of vat versus continuous heating systems of milk. J Dairy Sci 69:2593–2603

    Article  Google Scholar 

  • Peshev D, van den Ende W (2014) Fructans: prebiotics and immunomodulators. J Funct Foods 8:348–357

    Article  CAS  Google Scholar 

  • Pfeffer MA, Frohlich ED (2006) Improvements in clinical outcomes with the use of angiotensin-converting enzyme inhibitors: cross-fertilization between clinical and basic investigation. Am J Physiol Heart Circ Physiol 291:H2021–H2025

    Article  CAS  PubMed  Google Scholar 

  • Pihlanto-Leppälä A (2001) Bioactive peptides derived from bovine whey proteins: opioid and ACE-inhibitory peptides. Trends Food Sci Technol 11:347–356

    Article  Google Scholar 

  • Power O, Jakeman P, FitzGerald RJ (2012) Antioxidative peptides: enzymatic production, in vitro and in vivo antioxidant activity and potential applications of milk-derived antioxidative peptides. Amino Acids 44:797–820

    Article  CAS  PubMed  Google Scholar 

  • Power-Grant O, McCormack WG, Ramia De Cap M, Amigo-Benavent M, Fitzgerald RJ, Jakeman P (2016) Evaluation of the antioxidant capacity of a milk protein matrix in vitro and in vivo in women aged 50–70 years. Int J Food Sci Nutr 67(3):325–334

    Article  CAS  PubMed  Google Scholar 

  • Rajasekaran A, Sivagnanam G, Xavier R (2008) Nutraceuticals as therapeutic agents: a review. Res J Pharm Technol 1(4):328–340

    CAS  Google Scholar 

  • Ramachandran L, Shah NP (2010) Characterization of functional, biochemical and textural properties of synbiotic low-fat yogurts during refrigerated storage. Food Sci Technol 43:819–827

    Google Scholar 

  • Rastall RA, Gibson GR, Gill HS, Guarner F, Klaenhammer TR, Pot B, Reid G, Rowland IR, Sanders ME (2005) Modulation of the microbial ecology of the human colon by probiotics, prebiotics and symbiotic to enhance human health: an overview of enabling science and potential applications. FEMS Microbiol Ecol 52:145–152

    Article  CAS  PubMed  Google Scholar 

  • Ravula RR, Shah NP (1998) Selective enumeration of Lactobacillus casei from yogurts and fermented milk drinks. Biotechnol Technol 12:819–822

    Article  CAS  Google Scholar 

  • Remeuf F, Mohammed S, Sodini I, Tissier JP (2003) Preliminary observations on the effects of milk fortification and heating on microstructure and physical properties of stirred yogurt. Int Dairy J 13:773–782

    Article  CAS  Google Scholar 

  • Ribeill YJ (2013) Discovery of new medicines. In: Griffin JP, Posner J, Barker GR (eds), The textbook of pharmaceutical medicine. Blackwell Publishing Ltd, Oxford

    Google Scholar 

  • Rokka T, Syväoja EL, Tuominen J, Korhonen H (1997) Release of bioactive peptides by enzymatic proteolysis of Lactobacillus GG fermented UHT-milk. Milchwissenschaft 52:675–678

    CAS  Google Scholar 

  • Sarmadi BH, Ismail A (2010) Antioxidative peptides from food proteins: a review. Peptides 31:1949–1956

    Article  CAS  PubMed  Google Scholar 

  • Serban DE (2013) Gastrointestinal cancers: influence of gut microbiota, probiotics and prebiotics. Cancer Lett 2013:1–13

    Google Scholar 

  • Shah NP (2007) Functional cultures and health benefits. Int Dairy J 17:1262–1277

    Article  Google Scholar 

  • Shakerian M, Razavi SH, Ziai SA, Khodaiyan F, Yarmand MS, Moayedi A (2015) Proteolytic and ACE-inhibitory activities of probiotic yogurt containing non-viable bacteria as affected by different levels of fat, inulin and starter culture. J Food Sci Technol 52(4):2428–2433

    Article  CAS  PubMed  Google Scholar 

  • Shihata A, Shah NP (2000) Proteolytic profiles of yogurt and probiotic bacteria. Int Dairy J 10:401–408

    Article  CAS  Google Scholar 

  • Sica DA (2004) Angiotensin-converting enzyme inhibitors side effects—physiologic and non-physiologic considerations. J Clini Hypertension 6:410–416

    Article  CAS  Google Scholar 

  • Sodini I, Mattas J, Tong PS (2006) Influence of pH and heat treatment of whey on the functional properties of whey protein concentrates in yoghurt. Int Dairy J 12:1464–1469

    Article  CAS  Google Scholar 

  • Stanton C, Ross RP, Fitzgerald GF, Sinderen DV (2005) Fermented functional foods based on probiotics and their biogenic metabolites. Curr Opin Biotechnol 16:198–203

    Article  CAS  PubMed  Google Scholar 

  • Sun J, He H, Xie B (2004) Novel antioxidant peptides from fermented mushroom Ganoderma lucidum. J Agric Food Chem 52:6646–6652

    Article  CAS  PubMed  Google Scholar 

  • Thompson AM, Hu T, Eshelbrenner CL, Reynolds K, He J, Bazzano LA (2011) Antihypertensive treatment and secondary prevention of cardiovascular disease events among persons without hypertension: a meta-analysis. J Am Med Assoc 305(9):913–922

    Article  CAS  Google Scholar 

  • Varadharaj S, Kelly OJ, Khayat RN, Kumar PS, Ahmed N, Zweier JL (2017) Role of dietary antioxidants in the preservation of vascular function and the modulation of health and disease. Front Cardiovasc Med 4(64):1–11

    Google Scholar 

  • Velez-Ruiz JF, Hernandez-Carranza P, Sosa-Morales M (2013) Physiochemical and flow properties of low-fat yogurt fortified with calcium and fiber. J Food Process Preserv 37(3):210–221

    Article  CAS  Google Scholar 

  • Villena J, Kitazawa H (2017) Probiotic microorganisms: a closer look. Microorganisms 5(17):1–3

    Google Scholar 

  • Vinderola CG, Bailo N, Reinheimer JA (2000) Survival of probiotic microflora in Argentinian yoghurts during refrigerated storage. Food Res Int 33:97–102

    Article  CAS  Google Scholar 

  • Vonk RJ, Reckman GAR, Harmsen HJM, Priebe MG (2012) Probiotics and lactose intolerance. In: Rigobelo EC (ed), Probiotics. Intech, Rijeka, pp 149–160

    Google Scholar 

  • Yuksel Z, Erdem Y (2010) The influence of transglutaminase treatment on functional properties of set yoghurt. Int J Dairy Technol 63:86–97

    Article  CAS  Google Scholar 

  • Zhao XH, Fu Y, Yue N (2014) In vitro cytoprotection of modified casein hydrolysates by plastein reaction on rat hepatocyte cells. CyTA J Food 12(1):40–47

    Article  CAS  Google Scholar 

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Acknowledgements

This work was financially supported by Ferdowsi University of Mashhad-Research Affairs (Contract No. 32051).

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  1. Department of Food Science and Technology, Faculty of Agriculture, Ferdowsi University of Mashhad, P. O. Box 91775-1163, Mashhad, Iran

    Mohammad B. Habibi Najafi, Saeideh Sadat Fatemizadeh & Maryam Tavakoli

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Habibi Najafi, M.B., Fatemizadeh, S.S. & Tavakoli, M. Release of Proteolysis Products with ACE-Inhibitory and Antioxidant Activities in Probiotic Yogurt Containing Different Levels of Fat and Prebiotics. Int J Pept Res Ther 25, 367–377 (2019). https://doi.org/10.1007/s10989-018-9679-8

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