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Quantification of Trypsin Activity by a New Biosensing System Based on the Enzymatic Degradation and the Destructive Nature of Trypsin

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Abstract

Trypsin is an enzyme that is included in the hydrolysis class of enzymes. Trypsin catalyzes the cleavage of proteins from a serine residue and is found in the digestive system of vertebrates. The cystic fibrosis, which is an autosomal recessive disorder, is associated with the trypsin deficiency. The meconium ileus is caused by this deficiency. Beside the clinical significance of trypsin, it is also used in many industrial applications, such as tissue culture labs, milk industry, proteomics, and different kinds of food production processes. In this study, a glucose oxidase based electrochemical biosensing system was constructed for sensitive and accurate determination of trypsin activity in different samples. A novel working principle was operated in the reported biosensor, which is based on the trypsin degradation ratio in the bioactive layer of the biosensor. Detailed optimization and characterization experiments were carried out to obtain the best electrochemical signals. The system introduced a linear determination range for trypsin activity between 6.65 and 33.25 U/ml. The biosensor was also used in the analysis of the trypsin activities of real samples.

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Acknowledgements

This study was financially supported by The Scientific and Technological Research Council of Turkey (TUBİTAK) in the frame of the project numbered 113Z678.

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  1. Biochemistry Division, Chemistry Department, Faculty of Science, Namık Kemal University, Tekirdağ, Turkey

    Belfin Işık & Mustafa Kemal Sezgintürk

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  1. Belfin Işık
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  2. Mustafa Kemal Sezgintürk
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Correspondence to Mustafa Kemal Sezgintürk.

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Belfin Işık and Mustafa Kemal Sezgintürk declare that they have no conflict of interest.

Informed Consent

All procedures followed were in accordance with the ethical standards of the responsible committee on human experimentation (institutional and national) and with the Helsinki Declaration of 1975, as revised in 2000 (5). Informed consent was obtained from all patients for being included in the study.

Human and Animal Rights

All institutional and national guidelines for the care and use of laboratory animals were followed. All procedures followed were in accordance with the ethical standards of the responsible committee on human experimentation (institutional and national) and with the Helsinki Declaration of 1975, as revised in 2008 (5). Informed consent was obtained from all patients for being included in the study.

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Işık, B., Sezgintürk, M.K. Quantification of Trypsin Activity by a New Biosensing System Based on the Enzymatic Degradation and the Destructive Nature of Trypsin. Int J Pept Res Ther 23, 313–322 (2017). https://doi.org/10.1007/s10989-016-9563-3

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