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1H-NMR-Based Metabonomics Study on the Restorative Effect of Soybean Polypeptide in Rats of Oxidative Damaged Induced by d-Galactose

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Abstract

Soybean polypeptide TTYY has substantial antioxidant activity. The present study aimed to investigate the mechanisms of the potential ability of TTYY to allow rats to recover from oxidative damage using 1H-NMR-based metabonomics. Male Wistar rats received injections of d-galactose to establish the oxidative damage model, then gavaged with TTYY. After treatment, principal component analysis and partial least squares-discriminant analysis were performed to identify different metabolic profiles based on the 1H-NMR spectra of urine from rats and liver antioxidant capacity analysis were also performed. The oxidation-induced changed in biological endpoints were reversed to a certain extent in the rats treated with TTYY compared with the control group. The potential biomarkers in the urine were identified as follows: pyruvate, 2-ketoglutarate, alanine, glycine, citrate, creatine, lactate, and acetate. The results show that TTYY may counterbalance the oxidative damage induced by d-galactose, and most likely plays a role in the changes observed in certain metabolic pathways including those involved in energy, amino acid, and glycolysis metabolisms.

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Funding

This study was funded by the National Project of Scientific and Technical Supporting Programs of China (Grant Number 2012bad33b03).

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Authors and Affiliations

  1. College of Food Science and Engineering, Jilin University, No. 5333, Xi’an Road, Changchun, 130000, Jilin, China

    Yuan Dai, Jingbo Liu & Zhongsu Ma

  2. School of Food Engineering, Jilin Business and Technology College, Changchun, 130000, China

    Jinsong Leng

  3. School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, 110016, China

    Haifeng Wang

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  1. Yuan Dai
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Correspondence to Jingbo Liu.

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Conflict of Interest

Yuan Dai, Jingbo Liu, Jinsong Leng, Zhongsu Ma, and Haifeng Wang declare that they have no conflict of interest.

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All applicable international guidelines for the care and use of animals were followed. This article does not contain any studies with human participants performed by any of the authors.

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Dai, Y., Liu, J., Leng, J. et al. 1H-NMR-Based Metabonomics Study on the Restorative Effect of Soybean Polypeptide in Rats of Oxidative Damaged Induced by d-Galactose. Int J Pept Res Ther 23, 37–47 (2017). https://doi.org/10.1007/s10989-016-9532-x

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