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Microcalorimetric study of the effect of manganese on the growth and metabolism in a heterogeneously expressing manganese-dependent superoxide dismutase (Mn-SOD) strain

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Abstract

In bacteria, manganese (Mn) is best understood for its roles in protection against oxidative stress as a cofactor of manganese-dependent superoxide dismutase (Mn-SOD). There are four SOD enzymes, including two distinct Mn-SOD proteins (SodA1 and SodA2), with an approximately 53% amino sequence identity to each other, one Cu/Zn-SOD and one Fe-SOD in Bacillus thuringiensis. The specific activity of heterogeneously expressed SodA1 enzyme in Escherichia coli was 10,860 U mg−1, which was enhanced with the addition of elevated exogenous Mn(II) levels and reached the highest specific activity (14,519 U mg−1) at 80 μM Mn(II). However, neither the purified SodA1 enzyme nor the E. coli recombinant strain BL21-SOD could oxidize Mn(II) in vitro or in vivo. The growth of BL21-SOD strain was also increased by 2 mM Mn(II), and its intracellular accumulated Mn(II) level reached 41.5 μM. The obtained power–time curves from microcalorimetric assay demonstrated that Q peak of BL21-SOD cultivated with 2 mM Mn(II) was significantly increased, which was 3.55-fold and 3.85-fold higher than the parent strain BL21(DE3) and control strain BL21-pET, respectively, indicating that the exposure of Mn(II) and accompanying oxidative stress might induce and activate the overproduction of SodA1 to eliminate toxic O −2 .

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant 31270105), the Fundamental Research Funds for the Central universities (Grant 2662015PY175), the National High-tech R&D Program of China (863 Program, Grant 2011AA10A205) and the National Basic Research Program of China (973 Program, Grant 2010CB126105).

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Authors and Affiliations

  1. State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People’s Republic of China

    Jianmei Su, Zhou Li, Bei Liao, Xiaodi Zhang & Jin He

  2. School of Health Sciences, Wuhan University, Wuhan, 430071, Hubei, People’s Republic of China

    Jianmei Su & Chunhong Wang

  3. Angel Yeast Co., Ltd., Yichang, 443003, Hubei, People’s Republic of China

    Bei Liao

  4. Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtse River), Ministry of Agriculture, College of Resources and Environment, Huazhong Agricultural University, Wuhan, 430070, Hubei, People’s Republic of China

    Yanhua Zhu

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  1. Jianmei Su
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Correspondence to Jin He.

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Su, J., Li, Z., Liao, B. et al. Microcalorimetric study of the effect of manganese on the growth and metabolism in a heterogeneously expressing manganese-dependent superoxide dismutase (Mn-SOD) strain. J Therm Anal Calorim 130, 1407–1416 (2017). https://doi.org/10.1007/s10973-017-6282-8

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