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Synthesis, radiolabeling, and investigation of bombesin-modified gadolinium nanoparticles as a potential SPECT/MRI agent for prostate cancer

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Abstract

This work proposes a multifunctional diagnostic probe that is combined with magnetic resonance imaging (MRI) and single-photon emission computed tomography (SPECT) for imaging prostate cancer. For this purpose, 99mTc-radiolabeled bombesin-conjugated gadolinium nanoparticles ([99mTc]Tc-GdO@SiO-BBN-DFO) were synthesized. The average hydrodynamic diameter of the nanoconjugate was about 200 nm. The radiolabeling yield of [99mTc]Tc-GdO@SiO-BBN-DFO NPs was over 90%. In vitro studies were performed using healthy and cancerous prostate cells through the MTT test and apoptosis assay. In conclusion [99mTc]Tc-GdO@SiO-BBN-DFO NPs have the potential to be a novel multifunctional imaging SPECT/MRI probe and a promising bimodal imaging agent in the future.

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Abbreviations

GdO-NPs:

Gadolinium oxide nanoparticles

GdO-SiO:

Silica coated gadolinium oxide nanoparticles

GdO-SiO-BBN:

Bombesin modified silica coated gadolinium oxide nanoparticles

GdO-SiO-BBN-DFO:

Deferoxamine conjugated GdO-SiO-BBN nanoparticles

[99mTc]Tc-GdO-SiO-BBN-DFO:

Radiolabeled-Nanoconjugate (99mTc-GdO-SiO-BBN-DFO)

[99mTc]Tc -GdO-SiO-BBN:

Radiolabeled-Nanoconjugate (99mTc-GdO-SiO-BBN)

[99mTc]Tc-DFO:

Radiolabeled-DFO

NPs:

Nanoparticles

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Acknowledgements

Authors thank Dr. Halil İbrahim Özdemir for MRI imaging of nanoconjugates in Ege University Radiology Department and Kadriye Buşra Karatay for assistance with HPLC analyses. The work paper presented as poster at International Nuclear Sciences and Technologies Conference (INSTEC-22, 19-22 October 2022).

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  1. Department of Nuclear Applications, Institute of Nuclear Sciences, Ege University, 35100, Bornova, Izmir, Türkiye

    T. S. Akkaya, E. Tutun, V. Yasakçı & P. Unak

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  1. T. S. Akkaya
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  2. E. Tutun
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Correspondence to P. Unak.

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Akkaya, T.S., Tutun, E., Yasakçı, V. et al. Synthesis, radiolabeling, and investigation of bombesin-modified gadolinium nanoparticles as a potential SPECT/MRI agent for prostate cancer. J Radioanal Nucl Chem 332, 4755–4766 (2023). https://doi.org/10.1007/s10967-023-08965-7

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