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In vitro enzymatic technique for biological quality control of 99mTc-ECD

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Abstract

99mTc-ethylene dicysteine diethyl ester (99mTc-ECD) is an established radiopharmaceutical used for brain perfusion imaging; however it does not have any recognized biological quality control to ascertain its in vivo efficacy. The present paper describes an in vitro enzymatic method that can be adapted as a regular biological quality control for ECD kit evaluation before its release for patient end use. The method involves reaction of 99mTc-ECD (< 74 MBq; 0.1 mg ECD) with pig liver esterase enzyme (≥ 20 mg/mL) yielding metabolites in quantitative yield post 1 h incubation. Methodology developed is precise for routine quality control analyses of ECD kits.

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Authors and Affiliations

  1. Radiopharmaceuticals Program, Board of Radiation and Isotope Technology, Navi-Mumbai, 400703, India

    Soumen Das, Anupam Mathur, Preethi Nair, R. Vanaja & S. S. Sachdev

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  1. Soumen Das
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  2. Anupam Mathur
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  3. Preethi Nair
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  4. R. Vanaja
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  5. S. S. Sachdev
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Correspondence to Anupam Mathur.

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Das, S., Mathur, A., Nair, P. et al. In vitro enzymatic technique for biological quality control of 99mTc-ECD. J Radioanal Nucl Chem 318, 1287–1295 (2018). https://doi.org/10.1007/s10967-018-6055-2

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  • DOI: https://doi.org/10.1007/s10967-018-6055-2

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