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Low gamma irradiation effects on protein profile, solubility, oxidation, scavenger ability and bioavailability of essential minerals in black and yellow Indian soybean (Glycine max L.) varieties

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Abstract

Effect of low doses of gamma irradiation (0.25, 0.5 and 1.0 kGy) on protein oxidation, profile, solubility, ROS scavenging and in vivo bioavailability of minerals in black (BS1) and yellow (BRAGG) soybean varieties were investigated. Increased oxidation, altered protein profile with decreased solubility was observed higher in BRAGG compared with BS1. The most significant ROS scavenging effect, antioxidant activity, least phytate content and improved bioavailability was found at 0.5 kGy in BS1 than BRAGG due to anthocyanins, and phenolics. Still 1.0 kGy is considered as toxicologically and microbiologically safe but it causes biochemical alterations and thus 0.5 kGy can be the optimum dose with enriched nutraceutical properties.

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Abbreviations

FDA:

Food and drug administration

PUFA:

Poly unsaturated fatty acids

CVD:

Cardio vascular diseases

BCA:

Bicinchoninic acid assay

DNPH:

Dinitro phenyl hydrazine

TPTZ:

2,4,6-Tripyridyl-S-triazine

FRAP:

Ferric ion reducing power

PEB:

Protein extraction buffer

DPPH:

1,1-Diphenyl-2-pycril-hydrazil

SDS-PAGE:

Sodium dodecyl sulphate-poly acrylamide gel electrophoresis

DNP:

Dinitro phenyl hydrazine

Tris:

Tris(hydroxymethyl)aminomethane

RSC:

Radical scavenging capacity

RT:

Room temperature

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Acknowledgments

We thank Dr. S. K. Lal for providing the samples. This study was supported by grant in aid for scientific research by Indian Agricultural Research Institute, New Delhi, India.

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Authors and Affiliations

  1. Division of Biochemistry, Indian Agricultural Research Institute, New Delhi, 110012, India

    Veda Krishnan, Archana Singh, Vinutha Thimmegowda, Anil Dahuja, Raj Deo Rai & Archana Sachdev

  2. Centre for Environment Science and Climate Resilient Agriculture (CESCRA), Indian Agricultural Research Institute, New Delhi, 110012, India

    Bhupinder Singh

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  1. Veda Krishnan
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Correspondence to Archana Sachdev.

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Krishnan, V., Singh, A., Thimmegowda, V. et al. Low gamma irradiation effects on protein profile, solubility, oxidation, scavenger ability and bioavailability of essential minerals in black and yellow Indian soybean (Glycine max L.) varieties. J Radioanal Nucl Chem 307, 49–57 (2016). https://doi.org/10.1007/s10967-015-4193-3

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  • DOI: https://doi.org/10.1007/s10967-015-4193-3

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