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Preparative-scale separation of ethyl esters of diethylenetriaminepentaacetic acid by flash chromatography

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Abstract

The penta ethyl ester of diethylenetriamine pentaacetic acid (DTPA) can be used as a prodrug for decorporation of radionuclides in the event of an internal radiation incidents with transuranic elements. A C18 chromatography purification method was successfully developed using a step elution profile to isolate 0.5–3 g of the four ethyl esters of DTPA. Less than 11 % of the isolated di-ethyl and tri-ethyl esters of DTPA, and less than 20 % of the tetra-ethyl ester of DTPA, had degraded in phosphate buffered saline over a 168 h period.

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Acknowledgments

This work was supported by NIAID contracts HHSN266200500045C and HHSN272201000030C.

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Authors and Affiliations

  1. Division of Molecular Pharmaceutics, Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599, USA

    Jonathan Fitzsimmons & Michael Jay

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  1. Jonathan Fitzsimmons
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  2. Michael Jay
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Correspondence to Jonathan Fitzsimmons or Michael Jay.

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Fitzsimmons, J., Jay, M. Preparative-scale separation of ethyl esters of diethylenetriaminepentaacetic acid by flash chromatography. J Radioanal Nucl Chem 305, 329–336 (2015). https://doi.org/10.1007/s10967-014-3904-5

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  • DOI: https://doi.org/10.1007/s10967-014-3904-5

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