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Preparation and characterization of oxaliplatin drug delivery vehicle based on PEGylated half-generation PAMAM dendrimer

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Abstract

Dendrimers were well-known as a polymeric nanoparticle drug carrier system. Among them, polyamidoamine (PAMAM) dendrimers were firstly and systematically studied. Herein, to explore the additional modification of PAMAM for drug deliver application, this study assessed the PEGylated half-generation G3.5 to load oxaliplatin (OXA). The proton nuclear magnetic resonance (1H NMR) and Fourier-transform infrared spectroscopy (FTIR) spectroscopy were used to confirm the successful synthesis of G3.5, and PEGylated G3.5. PEG modification on G3.5 neutralized the negative charge of G3.5 that was confirmed by zeta potential measurement, and increased the dimension of G3.5 from 10 to 100 nm that was carried out by TEM technique. G3.5-PEG showed the high drug loading efficiency of 75.69%. The release kinetic of OXA from G3.5-PEG@OXA indicated that no burst released phenomenon occurred (11.95% within first hour) and sustainable release was achieved. In cytotoxicity test with normal cells of L929 fibroblasts, the carrier system of G3.5-PEG did not induced any cytotoxicity. To test the killing effect of G3.5-PEG@OXA on cancerous cells of human cervical cancer cells (HeLa), lung adenocarcinoma (A549), and breast cancer (MCF-7), resazurin test and live/dead staining assay was used to observe the alive cells. The increase of OXA amount in G3.5-PEG@OXA lead to decrease the cell viability from 79.90–56.97% (HeLa), 84.80–64.00% (A549), and 92.00–65.00% (MCF-7) after 48 h treatment.

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Author notes

  1. Dinh Tien Dung Nguyen and Long Giang Bach contributed equally to this work

Authors and Affiliations

  1. Vietnam Academy of Science and Technology, Graduate University of Science and Technology, Hanoi, Vietnam

    Dinh Tien Dung Nguyen, Minh Nhat Ho, Dai Hai Nguyen & Cuu Khoa Nguyen

  2. Vietnam Academy of Science and Technology, Institute of Applied Materials Science, 01 TL29 District 12, Ho Chi Minh City, 70000, Vietnam

    Dinh Tien Dung Nguyen, Minh Nhat Ho, Dai Hai Nguyen & Cuu Khoa Nguyen

  3. Nguyen Tat Thanh University, 300A Nguyen Tat Thanh, District 4, Ho Chi Minh City, 700000, Vietnam

    Long Giang Bach

  4. International University, Vietnam National University-Ho Chi Minh City (VNU-HCMC), Ho Chi Minh City, 700000, Viet Nam

    Thi Hiep Nguyen & Minh Hieu Ho

  5. Biomaterials and Nanotechnology Research Group, Faculty of Applied Sciences, Ton Duc Thang University, Ho Chi Minh City, Vietnam

    Thai Thanh Hoang Thi

Authors
  1. Dinh Tien Dung Nguyen
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  2. Long Giang Bach
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Correspondence to Thai Thanh Hoang Thi.

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Nguyen, D.T.D., Bach, L.G., Nguyen, T.H. et al. Preparation and characterization of oxaliplatin drug delivery vehicle based on PEGylated half-generation PAMAM dendrimer. J Polym Res 26, 116 (2019). https://doi.org/10.1007/s10965-019-1779-4

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  • DOI: https://doi.org/10.1007/s10965-019-1779-4

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