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Evaluation of the bacterial anti-adhesive properties of polyacrylic acid, chitosan and heparin-modified medical grade Silicone rubber substrate

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Abstract

In this study, we aimed to develop polyelectrolyte multilayered films that could be absorbed onto a silicone rubber substrate; this composite material was then tested for its ability to inhibit bacterial adhesion. Polyacrylic acid (PAA) grafting onto the silicone rubber substrate was confirmed by attenuated total reflectance-infrared spectroscopy and the surface roughness of multilayered films was measured using an atomic force microscope and scanning electron microscopy. Immobilization of PAA, chitosan (CS) and heparin (HEP) films conferred antibacterial activity against Escherichia coli and Staphylococcus Aureus. The adhesion of both E. coli and S. aureus on PAA-CS-HEP multilayer film was reduced when compared to pristine, PAA, and PAA–CS films; this was due to effective complex formation between polyelectrolytes and cell adhesion proteins. Moreover, immobilization of HEP on the PAA-CS composite was efficiently reduced cell adhesion.

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Acknowledgments

The authors would like to thank the Armed Forces Taoyuan General Hospital (Project numbers: 10220) for financially supporting and opinions counseling in this work.

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    Authors and Affiliations

    1. Department of Community Medicine Family Medicine, Armed Forces Taoyuan General Hospital, Taoyuan, 325, Taiwan, Republic of China

      Yung-Zung Chang, Jing-Tang Lin & Po-Chung Chen

    2. Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei, 106, Taiwan, Republic of China

      Adhimoorthy Prasannan & Hsieh-Chih Tsai

    3. Department of Community Medicine, Armed Forces Taoyuan General Hospital, Taoyuan, 325, Taiwan, Republic of China

      Chou-Yuan Ko

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    1. Yung-Zung Chang
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    2. Jing-Tang Lin
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    Correspondence to Hsieh-Chih Tsai.

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    Yung-Zung Chang and Jing-Tang Lin contributed equally to this work.

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    Chang, YZ., Lin, JT., Prasannan, A. et al. Evaluation of the bacterial anti-adhesive properties of polyacrylic acid, chitosan and heparin-modified medical grade Silicone rubber substrate. J Polym Res 22, 131 (2015). https://doi.org/10.1007/s10965-015-0767-6

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    • DOI: https://doi.org/10.1007/s10965-015-0767-6

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