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Luteolin-Loaded Spion as a Drug Carrier for Cancer Cell In Vitro

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Abstract

The aim of this study is to determine the cytotoxic and apoptotic effects of newly synthesized folic acid (FA) and luteolin (carboxylated luteolin (CL)) conjugated superparamagnetic nanoparticle (SPION@FA-PEG@CL) on folate receptor positive (FAR (+)) and negative (FAR (−)) cell lines in vitro. Cytotoxicity analysis by real-time cell analyzer system (RTCA) and apoptosis by TUNEL and Annexin assay, Caspase 3/7 activities, and Caspase 3/7 expression level were determined. According to RTCA results, SPION@FA-PEG@CL nanodrug showed higher cytotoxicity against U87, MCF-7, HeLa cell lines in comparison with L929 and A549 cell lines. TUNEL and Annexin assays indicated that SPION@FA-PEG@CL increased the apoptotic and necrotic cell ratio in FAR (+) cell. In L929 and A549 cells, are FAR (−) cell lines apoptotic and necrotic cell ratio was lower than FAR (+) cell lines (U87, MCF-7 and HeLa). Caspase 3 and 7 analyses also showed that SPION@FA-PEG@CL nanodrug have apoptotic and necrotic impact on cell lines, they were higher in FAR (+) cells than FAR (−) cells. According to results, synthesized SPION@FA-PEG@CL can be a novel nanodrug delivery system for in cancer chemotherapy.

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Acknowledgements

The authors are thankful to the TÜBİTAK, Research Project Foundation (Contract no: 114Z581 for financial support of this study.

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Authors and Affiliations

  1. Gama Mechatronics Company, 16159, Nilüfer-Bursa, Turkey

    L. Alpsoy & Z. Ü. Akal

  2. Department of Nano-Medicine Research, Institute for Research & Medical Consultations (IRMC), University of Dammam, P.O. Box 1982, Dammam, 31441, Saudi Arabia

    A. Baykal

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  1. L. Alpsoy
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Correspondence to A. Baykal.

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Alpsoy, L., Baykal, A. & Akal, Z.Ü. Luteolin-Loaded Spion as a Drug Carrier for Cancer Cell In Vitro. J Supercond Nov Magn 31, 467–474 (2018). https://doi.org/10.1007/s10948-017-4199-x

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