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Identification and Functional Analysis of Novel Bradykinin-Related Peptides (BRPs) from Skin Secretions of Five Asian Frogs

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Abstract

In recent decades, various types of bioactive substances have been identified from amphibian skin and its secretions. Bradykinin-related peptides (BRPs) are among these compounds that make up the host defence system of amphibians. In the present study, we identified six novel BRPs, amolopkinin-GN1, amolopkinin-RK1, amolopkinin-TR1, amolopkinin-LF1, ranakinin-MS1, and ranakinin-MS2, from five East Asian amphibians, Amolops granulosus, Amolops ricketti, Amolops torrentis, Amolops lifanensis, and Hylarana maosonensis. This is the first report on BRPs in the skin of these species. Physiological assays reveal that these peptides have a contractive effect on the smooth muscle of rat ileum.

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Funding

The study was supported by the Natural Science Foundation of Educational Committee of Hebei Province of China (Nos. QN2016129), Natural Science Foundation of Hebei Province of China (Nos. C2016205244), and National Natural Science Foundation of China (Nos. 31200309).

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Author notes

  1. Baowen Zhang and Xiaoli Zhang have contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Sciences, Hebei Normal University, Shijiazhuang, 050024, Hebei, China

    Baowen Zhang, Xiaoli Zhang, Ying Yang & Hui Wang

  2. Instrumental Analysis Center, Hebei Normal University, Shijiazhuang, 050024, Hebei, China

    Yuhong Hu

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  1. Baowen Zhang
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  2. Xiaoli Zhang
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  3. Ying Yang
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  4. Yuhong Hu
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  5. Hui Wang
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Corresponding authors

Correspondence to Yuhong Hu or Hui Wang.

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All authors declare that they have no conflicts of interest.

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Zhang, B., Zhang, X., Yang, Y. et al. Identification and Functional Analysis of Novel Bradykinin-Related Peptides (BRPs) from Skin Secretions of Five Asian Frogs. Protein J 37, 324–332 (2018). https://doi.org/10.1007/s10930-018-9783-9

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  • DOI: https://doi.org/10.1007/s10930-018-9783-9

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