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Development of Insoluble PVA Electrospun Nanofibers Incorporating R-Limonene or β-Cyclodextrin/R-Limonene Inclusion Complexes

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Abstract

Electrospinning of water-soluble polymers is considered a green process for encapsulating active compounds in polymeric nanofibers, but generally the obtained materials remain water-soluble so applications are limited. In this paper, water-insoluble poly(vinyl alcohol) (PVA) nanofibers incorporating R-limonene (a highly volatile antimicrobial) were developed. In order to avoid R-limonene loses during fabrication, β-cyclodextrin was added to the formulation. A proper heat treatment of the materials led to water-insolubilization by means of citric acid crosslinking. Nanofibers diameters were increased by the presence of β-cyclodextrin/R-limonene as a consequence of new crystalline structure. Also, encapsulation efficiency of R-limonene was improved from (19 ± 1)% to (70 ± 1)% when β-cyclodextrin was present in the formulation. Antimicrobial activity resulted low; even so, nanofibers incorporating R-limonene inhibited Escherichia coli growth. Biodegradation rate of PVA nanofibers decreased significantly when incorporating R-limonene, but it was unaffected when the active principle was also encapsulated in β-cyclodextrin. The results of this research showed that an additional encapsulation of volatile compounds with cyclodextrins could be useful to improve encapsulation efficiency in heat-treatment crosslinked materials while maintaining both the antimicrobial activity and biodegradable character of the material.

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Funding

This work was supported by the following organizations: CONICET (Grant Nos. PIP 2014–2016, 11220120100508CO), University of Buenos Aires (Grant Nos. UBACYT2014–2017, 20020130100495BA), ANPCyT (Grant Nos. PICT-2012-1093, PICT 2016-2940).

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Authors and Affiliations

  1. Departamento de Física, Laboratorio de Polímeros y Materiales Compuestos (LP&MC), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Instituto de Física de Buenos Aires (IFIBA-CONICET), Ciudad Universitaria, 1428, Buenos Aires, Argentina

    Santiago Estevez-Areco, Lucas Guz & Silvia Goyanes

  2. Instituto de Investigación e Ingeniería Ambiental, CONICET, Universidad Nacional de San Martín, Campus Miguelete, 25 de mayo y Francia, 1650, San Martín, Pcia Buenos Aires, Argentina

    Roberto Candal

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  1. Santiago Estevez-Areco
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  2. Lucas Guz
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  3. Roberto Candal
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  4. Silvia Goyanes
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Correspondence to Roberto Candal or Silvia Goyanes.

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Estevez-Areco, S., Guz, L., Candal, R. et al. Development of Insoluble PVA Electrospun Nanofibers Incorporating R-Limonene or β-Cyclodextrin/R-Limonene Inclusion Complexes. J Polym Environ 30, 2812–2823 (2022). https://doi.org/10.1007/s10924-022-02390-9

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