Skip to main content
Log in

Preparation of Active Nanocomposite Film Consisting of Sodium Caseinate, ZnO Nanoparticles and Rosemary Essential Oil for Food Packaging Applications

  • Original Paper
  • Published:
Journal of Polymers and the Environment Aims and scope Submit manuscript

Abstract

Active films based on sodium caseinate (SC) were fabricated via the casting method; these films were reinforced with zinc oxide nanoparticles (ZnO NPs) and rosemary essential oil (REO). The films’ optical, mechanical, and barrier and antimicrobial properties were evaluated. Further characterization was done using SEM, XRD, and FTIR spectroscopy. The results disclosed that the incorporation of ZnO NPs and REO into the SC matrix significantly improved the mechanical and barrier properties as well as moisture resistance of the SC film. The simultaneous incorporation of ZnO NPs and REO considerably reduced the water vapor permeability (WVP) of the films but increased their flexibility and strength. The microscopic and spectroscopic characterization revealed that the additives were highly compatible with the SC film matrix given the formation of uniform and homogeneous composite films. The nanocomposite films also exhibited noteworthy antimicrobial activity against the tested bacteria. In summary, this type of biopolymer-based active film can be considered as a promising material for food packaging applications.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

Abbreviations

SEM:

Scanning electron microscopy

XRD:

X-ray diffraction

FTIR:

Fourier transform infrared

WVP:

Water vapor permeability

ZnO:

Zinc oxide

NPs:

Nanoparticles

SC:

Sodium caseinate

REO:

Rosemary essential oil

References

  1. Tankhiwale R, Bajpai SK (2012) Preparation, characterization and antibacterial applications of ZnO-nanoparticles coated polyethylene films for food packaging. Coll Surf B: Biointerfaces 90:16–20

    CAS  Google Scholar 

  2. Bagheri V, Ghanbarzadeh B, Ayaseh A, Ostadrahimi A, Ehsani A, Alizadeh-Sani M, Adun PA (2019) The optimization of physico-mechanical properties of bionanocomposite films based on gluten/carboxymethyl cellulose/cellulose nanofiber using response surface methodology. Poly Test 78:105989

    Google Scholar 

  3. Umamaheswari G, Sanuja S, John VA, Kanth SV, Umapathy MJ (2015) Preparation, characterization and anti-bacterial activity of zinc oxide-gelatin nanocomposite film for food packaging applications. Polym Polym Compos 23(3):199–204

    Google Scholar 

  4. Alizadeh-Sani M, Ehsani A, Kia EM, Khezerlou A (2019) Microbial gums: introducing a novel functional component of edible coatings and packaging. Appl Microbiol Biotechnol 103(17):6853–6866

    CAS  PubMed  Google Scholar 

  5. Piñeros-Hernandez D, Medina-Jaramillo C, López-Córdoba A, Goyanes S (2017) Edible cassava starch films carrying rosemary antioxidant extracts for potential use as active food packaging. Food Hydrocoll 63:488–495

    Google Scholar 

  6. Alizadeh-Sani M, Mohammadian E, McClements DJ (2020) Eco-friendly active packaging consisting of nanostructured biopolymer matrix reinforced with TiO2 and essential oil: application for preservation of refrigerated meat. Food Chem. https://doi.org/10.1016/j.foodchem.2020.126782

    Article  PubMed  Google Scholar 

  7. Alizadeh-Sani M, Rhim J-W, Azizi-Lalabadi M, Hemmati-Dinarvand M, Ehsani A (2020) Preparation and characterization of functional sodium caseinate/guar gum/TiO2/cumin essential oil composite film. Int J Biol Macromol 145:835–844

    CAS  PubMed  Google Scholar 

  8. Moghaddas Kia E, Ghasempour Z, Alizadeh M (2018) Fabrication of an eco-friendly antioxidant biocomposite: Zedo gum/sodium caseinate film by incorporating microalgae (Spirulina platensis). J Appl Polym Sci 135(13):46024

    Google Scholar 

  9. Fabra MJ, Talens P, Chiralt A (2008) Tensile properties and water vapor permeability of sodium caseinate films containing oleic acid–beeswax mixtures. J Food Eng 85(3):393–400

    CAS  Google Scholar 

  10. Suppakul P, Miltz J, Sonneveld K, Bigger SW (2003) Active Packaging technologies with an emphasis on antimicrobial packaging and its applications. J Food Sci 68(2):408–420

    CAS  Google Scholar 

  11. Fabra MJ, Talens P, Chiralt A (2009) Microstructure and optical properties of sodium caseinate films containing oleic acid–beeswax mixtures. Food Hydrocoll 23(3):676–683

    CAS  Google Scholar 

  12. Kia EM, Ghasempour Z, Ghanbari S, Pirmohammadi R, Ehsani A (2018) Development of probiotic yogurt by incorporation of milk protein concentrate (MPC) and microencapsulated Lactobacillus paracasei in gellan-caseinate mixture. Br Food J. https://doi.org/10.1108/BFJ-12-2017-0668

    Article  Google Scholar 

  13. Sani MA, Ehsani A, Hashemi M (2017) Whey protein isolate/cellulose nanofibre/TiO2 nanoparticle/rosemary essential oil nanocomposite film: its effect on microbial and sensory quality of lamb meat and growth of common foodborne pathogenic bacteria during refrigeration. Int J Food Microbiol 251:8–14

    Google Scholar 

  14. Ojeda-Sana AM, van Baren CM, Elechosa MA, Juárez MA, Moreno S (2013) New insights into antibacterial and antioxidant activities of rosemary essential oils and their main components. Food cont 31(1):189–195

    CAS  Google Scholar 

  15. Li K-K, Yin S-W, Yang X-Q, Tang C-H, Wei Z-H (2012) Fabrication and characterization of novel antimicrobial films derived from thymol-loaded zein–sodium caseinate (SC) nanoparticles. J Agricul Food Chem 60(46):11592–11600

    CAS  Google Scholar 

  16. Al-Naamani L, Dobretsov S, Dutta J (2016) Chitosan-zinc oxide nanoparticle composite coating for active food packaging applications. Innovat Food Sci Emerg Technol 38:231–237

    CAS  Google Scholar 

  17. Alizadeh-Sani M, Khezerlou A, Ehsani A (2018) Fabrication and characterization of the bionanocomposite film based on whey protein biopolymer loaded with TiO2 nanoparticles, cellulose nanofibers and rosemary essential oil. Indust Crops Prod 124:300–315

    CAS  Google Scholar 

  18. ASTM-D1746-09 (1977) Annual Book of ASTM. American Society for Testing and Materials, Philadelphia, PA

  19. ASTM-E96 (1995) Annual Book of ASTM. American Society for Testing and Materials, Philadelphia, PA

  20. Alswat AA, Ahmad MB, Saleh TA, Hussein MZB, Ibrahim NA (2016) Effect of zinc oxide amounts on the properties and antibacterial activities of zeolite/zinc oxide nanocomposite. Mater Sci Eng: C 68:505–511

    CAS  Google Scholar 

  21. Luo Z, Qin Y, Ye Q (2015) Effect of nano-TiO2-LDPE packaging on microbiological and physicochemical quality of Pacific white shrimp during chilled storage. Int J Food Sci Technol 50(7):1567–1573

    CAS  Google Scholar 

  22. Alizadeh-Sani M, Hamishehkar H, Khezerlou A, Maleki M, Azizi-Lalabadi M, Bagheri V, Safaei P, Azimi T, Hashemi M, Ehsani A (2020) Kinetics analysis and susceptibility coefficient of the pathogenic bacteria by titanium dioxide and zinc oxide nanoparticles. Adv Pharmac Bull 10(1):56

    CAS  Google Scholar 

  23. Ando H, Kawasaki N, Yamano N, Uegaki K, Nakayama A (2015) Biodegradation of a poly (ε-caprolactone-co-l-lactide)–visible-light-sensitive TiO2 composite with an on/off biodegradation function. Polym Degrad Stabil 114:65–71

    CAS  Google Scholar 

  24. Azizi-Lalabadi M, Ehsani A, Alizadeh-Sani M, Khezerlou A, Mirzanajafi-Zanjani M, Divband B, Zolfaghari H, Bagheri V (2019) Nanoparticles and zeolites: antibacterial effects and their mechanism against pathogens. Curr Pharmac Biotechnol 20(13):1074–1086

    CAS  Google Scholar 

  25. Choi WS, Singh S, Lee YS (2016) Characterization of edible film containing essential oils in hydroxypropyl methylcellulose and its effect on quality attributes of ‘Formosa’plum (Prunus salicina L.). LWT-Food Science and Technology 70:213–222

    CAS  Google Scholar 

  26. Arfat YA, Benjakul S, Prodpran T, Sumpavapol P, Songtipya P (2014) Properties and antimicrobial activity of fish protein isolate/fish skin gelatin film containing basil leaf essential oil and zinc oxide nanoparticles. Food Hydrocoll 41:265–273

    CAS  Google Scholar 

  27. Alebooyeh R, MohammadiNafchi A, Jokr M (2018) The effects of ZnOnanorodson the characteristics of sago starch biodegradable films. J Chem Health Risks 2(4)

  28. Kuang Q, Xu J, Liang Y, Xie F, Tian F, Zhou S, Liu X (2017) Lamellar structure change of waxy corn starch during gelatinization by time-resolved synchrotron SAXS. Food Hydrocoll 62:43–48

    CAS  Google Scholar 

  29. Almasi H, Ghanbarzadeh B, Entezami AA (2010) Physicochemical properties of starch–CMC–nanoclay biodegradable films. Int J Biol Macromol 46(1):1–5

    CAS  PubMed  Google Scholar 

  30. Zolfi M, Khodaiyan F, Mousavi M, Hashemi M (2014) Development and characterization of the kefiran-whey protein isolate-TiO2 nanocomposite films. Int J Biol Macromol 65:340–345

    CAS  PubMed  Google Scholar 

  31. Zolfi M, Khodaiyan F, Mousavi M, Hashemi M (2014) The improvement of characteristics of biodegradable films made from kefiran–whey protein by nanoparticle incorporation. Carbohydr Polym 109:118–125

    CAS  PubMed  Google Scholar 

  32. Hosseini SF, Rezaei M, Zandi M, Farahmandghavi F (2015) Fabrication of bio-nanocomposite films based on fish gelatin reinforced with chitosan nanoparticles. Food Hydrocoll 44:172–182

    CAS  Google Scholar 

  33. El-Wakil NA, Hassan EA, Abou-Zeid RE, Dufresne A (2015) Development of wheat gluten/nanocellulose/titanium dioxide nanocomposites for active food packaging. Carbohydr Polym 124:337–346

    CAS  PubMed  Google Scholar 

  34. Zhang Y, Niu Y, Luo Y, Ge M, Yang T, Yu LL, Wang Q (2014) Fabrication, characterization and antimicrobial activities of thymol-loaded zein nanoparticles stabilized by sodium caseinate–chitosan hydrochloride double layers. Food Chem 142:269–275

    CAS  PubMed  Google Scholar 

  35. Wang L-J, Yin Y-C, Yin S-W, Yang X-Q, Shi W-J, Tang C-H, Wang J-M (2013) Development of novel zein-sodium caseinate nanoparticle (ZP)-stabilized emulsion films for improved water barrier properties via emulsion/solvent evaporation. J Agricul Food Chem 61(46):11089–11097

    CAS  Google Scholar 

  36. Pereda M, Amica G, Rácz I, Marcovich NE (2011) Structure and properties of nanocomposite films based on sodium caseinate and nanocellulose fibers. J Food Eng 103(1):76–83

    CAS  Google Scholar 

  37. Rouhi J, Mahmud S, Naderi N, Ooi CR, Mahmood MR (2013) Physical properties of fish gelatin-based bio-nanocomposite films incorporated with ZnO nanorods. Nanoscale Res Lett 8(1):364

    PubMed  PubMed Central  Google Scholar 

  38. Zhou J, Wang S, Gunasekaran S (2009) Preparation and characterization of whey protein film incorporated with TiO2 nanoparticles. J Food Sci 74(7):N50–N56

    CAS  PubMed  Google Scholar 

  39. Zhou J, Wang S, Gunasekaran S (2009) Preparation and characterization of whey protein film incorporated with TiO2 nanoparticles. J Food Sci 74(7)

  40. Li Y, Jiang Y, Liu F, Ren F, Zhao G, Leng X (2011) Fabrication and characterization of TiO 2/whey protein isolate nanocomposite film. Food Hydrocoll 25(5):1098–1104

    CAS  Google Scholar 

  41. Zolfi M, Khodaiyan F, Mousavi M, Hashemi M (2014) Development and characterization of the kefiran-whey protein isolate-TiO 2 nanocomposite films. Int J Biol Macromol 65:340–345

    CAS  PubMed  Google Scholar 

  42. Mergler Y, Schaake R (2004) Relation between strain hardening and wear resistance of polymers. J Appl Polym Sci 92(4):2689–2692

    CAS  Google Scholar 

  43. Sun L, Gibson RF, Gordaninejad F, Suhr J (2009) Energy absorption capability of nanocomposites: a review. Compos Sci Technol 69(14):2392–2409

    CAS  Google Scholar 

  44. Todorov L, Viana J (2007) Characterization of PET nanocomposites produced by different melt-based production methods. J Appl Polym Sci 106(3):1659–1669

    CAS  Google Scholar 

  45. Limpisophon K, Tanaka M, Osako K (2010) Characterisation of gelatin–fatty acid emulsion films based on blue shark (Prionace glauca) skin gelatin. Food Chem 122(4):1095–1101

    CAS  Google Scholar 

  46. Tongnuanchan P, Benjakul S, Prodpran T (2013) Physico-chemical properties, morphology and antioxidant activity of film from fish skin gelatin incorporated with root essential oils. J Food Eng 117(3):350–360

    CAS  Google Scholar 

  47. Yamada T, Hao L, Tada K, Konagaya S, Li G (2006) Crystallization characteristics of PET/TiO2 nanocomposites. Mater Scie 2:154–160

    CAS  Google Scholar 

  48. Shankar S, Teng X, Li G, Rhim J-W (2015) Preparation, characterization, and antimicrobial activity of gelatin/ZnO nanocomposite films. Food Hydrocoll 45:264–271

    CAS  Google Scholar 

  49. Mohanapriya S, Mumjitha M, Purnasai K, Raj V (2016) Fabrication and characterization of poly (vinyl alcohol)-TiO2 nanocomposite films for orthopedic applications. Journal of the mechanical behavior of biomedical materials 63:141–156

    CAS  PubMed  Google Scholar 

  50. Muyonga J, Cole C, Duodu K (2004) Characterisation of acid soluble collagen from skins of young and adult Nile perch (Lates niloticus). Food Chem 85(1):81–89

    CAS  Google Scholar 

  51. Nikoo M, Xu X, Benjakul S, Xu G, Ramirez-Suarez JC, Ehsani A, Kasankala LM, Duan X, Abbas S, (2011) Characterization of gelatin from the skin of farmed Amur sturgeon Acipenser schrenckii. Int Aquat Res (Islamic Azad University, Tonekabon Branch) 3(2)

Download references

Acknowledgements

The authors are grateful to Maragheh University of Medical Sciences for funding this work.

Author information

Authors and Affiliations

Authors

Corresponding authors

Correspondence to Ehsan Moghaddas Kia or Ali Ehsani.

Ethics declarations

Conflict of interest

The authors declare no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Alizadeh-Sani, M., Moghaddas Kia, E., Ghasempour, Z. et al. Preparation of Active Nanocomposite Film Consisting of Sodium Caseinate, ZnO Nanoparticles and Rosemary Essential Oil for Food Packaging Applications. J Polym Environ 29, 588–598 (2021). https://doi.org/10.1007/s10924-020-01906-5

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10924-020-01906-5

Keywords

Navigation