Abstract
In this work, we have studied a single molecule enzyme catalysis reaction in presence of oscillatory substrate concentration. The stochastic kinetics is modelled in terms of a chemical master equation. Depending on the oscillation frequency, hysteresis can occur in the system which is dynamic in nature. The time-dependent driving keeps the system out-of-equilibrium associated with dissipation. The interplay between the timescales of the system kinetics and the external driving necessitates the splitting of the total entropy production rate into adiabatic and nonadiabatic contributions. Analyses of these quantities give insights into the various balance conditions of the reaction fluxes and their roles in governing the nonequilibrium thermodynamics of the system. Interestingly, the net velocity of catalysis and the dissipation along with its various parts are found to exhibit hysteresis that vanish in the low and high-frequency ranges of substrate oscillation. However, the average (over a period) velocity as well as the average dissipation show hyperbolic increase with frequency to saturation. We have proposed an experimental protocol to realize such features using periodic stepwise injection of substrate at specified rates.
Similar content being viewed by others
References
H.P. Lu, L. Xun, X.S. Xie, Science 282, 1877 (1998)
H. Wang, G. Oster, Nature 396, 279 (1998)
G. Hummer, A. Szabo, Proc. Natl. Acad. Sci. USA 98, 3658 (2001)
F. Ritort, C. Bustamante, I. Tinoco Jr, Proc. Natl. Acad. Sci. USA 99, 13544 (2002)
D. Collin, F. Ritort, C. Jarzynski, S.B. Smith, I. Tinoco Jr, C. Bustamante, Nature 437, 231 (2005)
F. Ritort, Adv. Chem. Phys. 137, 31 (2008)
C. Jarzynski, Nat. Phys. 7, 591 (2011)
T. Xia, N. Li, X. Fang, Annu. Rev. Phys. Chem. 64, 459 (2013)
H. Qian, E.L. Elson, Biophys. Chem. 101–102, 565 (2002)
S.C. Kou, B.J. Cherayil, W. Min, B.P. English, X.S. Xie, J. Phys. Chem. B 109, 19068 (2005)
R. Grima, J. Chem. Phys. 133, 035101 (2010)
A.F. Bartholomay, Biochemistry 1, 223 (1962)
D.A. McQuarrie, J. Chem. Phys. 38, 433 (1963)
D.A. McQuarrie, C.J. Jachimowski, M.E. Russell, J. Chem. Phys. 40, 2914 (1964)
D.T. Gillespie, Phys. A 188, 404 (1992)
B. Das, G. Gangopadhyay, J. Chem. Phys. 132, 135102 (2010)
T.G. Kurtz, J. Chem. Phys. 57, 2976 (1972)
P. Aranyi, J. Toth, Acta Biochim. Biophys. Acad. Sci. Hung. 12, 375 (1977)
H. Qian, Annu. Rev. Phys. Chem. 58, 113 (2007)
R. Grima, J. Chem. Phys. 132, 185102 (2010)
A. Cornish-Bowden, Fundamentals of Enzyme Kinetics (Portland Press, London, 2004)
K. A. Johnson, R. S. Goody, Biochem. 50, 8264 (2011) (Translation of the 1913 Michaelis–Menten Paper)
B.P. English, W. Min, A.M. van Oijen, K.T. Lee, G. Luo, H. Sun, B.J. Cherayil, S.C. Kou, X.S. Xie, Nat. Chem. Biol. 2, 87 (2006)
C.Y. Mou, J.-L. Luo, G. Nicolis, J. Chem. Phys. 84, 7011 (1986)
K.L.C. Hunt, P.M. Hunt, J. Ross, Phys. A 147, 48 (1987)
P. Gaspard, J. Chem. Phys. 120, 8898 (2004)
T. Schmiedl, U. Seifert, J. Chem. Phys. 126, 044101 (2007)
J. Schnakenberg, Rev. Mod. Phys. 48, 571 (1976)
M. Esposito, C. Van den Broeck, Phys. Rev. Lett. 104, 090601 (2010)
H. Ge, H. Qian, Phys. Rev. E 81, 051133 (2010)
K. Banerjee, J. Math. Chem. 52, 2259 (2014)
R.K.P. Zia, B. Schmittmann, J. Phys. A 39, 407 (2006)
H. Ge, M. Qian, H. Qian, Phys. Rep. 510, 87 (2012)
L. Jiu-li, C. Van den Broeck, G. Nicolis, Z. Phys. B 56, 165 (1984)
W. Min, L. Jiang, J. Yu, S.C. Kou, H. Qian, X.S. Xie, Nano Lett. 5, 2373 (2005)
K. Banerjee, K. Bhattacharyya, Chem. Phys. 438, 1 (2014)
S.R. de Groot, P. Mazur, Non-equilibrium Thermodynamics (North-Holland, Amsterdam, 1962)
B.K. Chakrabarty, M. Acharyya, Rev. Mod. Phys. 71, 847 (1999)
M. Das, D. Mondal, D.S. Ray, J. Chem. Phys. 136, 114104 (2012)
M.A. Pustovoit, A.M. Berezhkovskii, S.M. Bezrukov, J. Chem. Phys. 125, 194907 (2006)
M. Esposito, C. Van den Broeck, Phys. Rev. E 82, 011143 (2010)
H. Ge, M. Qian, H. Qian, Phys. Rep. 510, 87 (2012)
D. Kondepudi, I. Prigogine, Modern Thermodynamics (from Engines to Dissipative Structures) (Wiley, New York, 2002)
Acknowledgments
K.B. acknowledges the University Grants Commission (UGC), India for Dr. D. S. Kothari Fellowship and Prof. K. Bhattacharyya for useful discussions.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Banerjee, K. Dynamic hysteresis in the velocity and dissipation of stochastic Michaelis–Menten kinetics. J Math Chem 53, 1412–1425 (2015). https://doi.org/10.1007/s10910-015-0496-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10910-015-0496-4