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Ecofriendly Approach for the Determination of Selected Aldehydes by Fluorescence Quenching of L-Tryptophan

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Abstract

It is a fluorescence-based study to examine the interaction between L-tryptophan and a selection of aldehydes, namely furfural (furan-2-carbaldehyde), 3-hydroxybenzaldehyde, salicylaldehyde (2-hydroxybenzaldehyde), 3-nitrobenzaldehyde, and 4-bromobenzaldehyde. The investigation took place in an aqueous environment, revealing that all five aldehydes induced quenching of the fluorescence intensity of L-tryptophan. By employing the Stern-Volmer equation to describe the quenching process, we constructed Stern-Volmer plots and derived Stern-Volmer constants. These constants (KSV) ranged from 2.87 × 104 mol L− 1 to 5.75 × 104 mol L− 1. Notably, the values of the Stern-Volmer constants varied among the different aldehydes, with the following order: 3-hydroxybenzaldehyde(3-HBA) > 4-bromobenzaldehyde (4-BBA) > 3-nitrobenzaldehyde > furan-2-carbaldehyde > salicylaldehyde. Consequently, our findings highlighted 3-hydroxybenzaldehyde as the most potent quencher, while 2-hydroxybenzaldehyde displayed the least sensitivity to quenching. Additionally, we determined the detection and quantification limits for the investigated aldehydes, resulting in ranges of 3.87 × 10− 12 to 8.25 × 10− 6 and 1.29 × 10− 11 to 2.75 × 10− 5, respectively. This research paves the way for the development of novel fluorescence probe-based sensors and offers valuable techniques for analyzing aldehydes within environmental and biological samples.

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Data Availability

Data has been included in the manuscript, though if the spectra of all the experiments performed was needed will be sent on request.

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Acknowledgements

The authors thank Allama Iqbal Open University Islamabad, Pakistan, for its assistance.

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  1. Department of Chemistry, Allama Iqbal Open University, Islamabad, 44000, Pakistan

    Muhammad Naeem Khan & Syeda Qandeel Wajid Kazmi

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  1. Muhammad Naeem Khan
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Contributions

Syeda Qandeel Wajid Kazmi prepared all the solutions, performed the experiment, and write the first draft of the manuscript. Muhammad Naeem Khan participated in the study design, the result and discussion, manuscript preparation, revision, and submission.

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Correspondence to Muhammad Naeem Khan.

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Khan, M.N., Kazmi, S.Q.W. Ecofriendly Approach for the Determination of Selected Aldehydes by Fluorescence Quenching of L-Tryptophan. J Fluoresc (2023). https://doi.org/10.1007/s10895-023-03456-0

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