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A Dual-mode Ratiometric Fluorometric and Colorimetric Platform Based on Nitrogen-doped Carbon Dots and o-phenylenediamine for the Detection of Nitrite

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Abstract

In this study, a dual-mode ratiometric fluorometric and colorimetric platform for the determination of nitrite in pickles was proposed by exquisitely employing the fact that non-fluorescent o-Phenylenediamine (OPD) was oxidized by nitrite under acidic conditions to form fluorescent 2,3-diaminophenazine (DAP) (Em = 575), which meanwhile quench the fluorescent nitrogen-doped carbon dots (N-CDs) at 455 nm, the ratio of fluorescence intensity of DAP to N-CDs (F575/F455) changed with the increase of nitrite accompanied by visible color changes. Thus, nitrite can be quantitatively detected within a wide linear range (10–500 µM) with a low detection limit of 0.45 µM due to the high quantum yield of 39.7% of N-CDs. In addition, the colour of the N-CDs/OPD system changed from transparent to yellow when the nitrite was introduced, enabling colorimetric and on-site visual detection. The detection limit of the colorimetric method was 3.03 µM with a linear range of 10–500 µM. The proposed ratiometric fluorometric method has pleasant selectivity and good immunity to interference.

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Funding

This research was supported by Zhejiang Provincial Natural Science Foundation of China (LY22C200008) and the National Natural Science Foundation of China (31772085).

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Authors and Affiliations

  1. Department of Applied Chemistry, College of Food Science and Biotechnology, Zhejiang Gongshang University, Xiasha University Town, No.18, Xuezheng St, Hangzhou, 310018, China

    Chendi Heng, Bowen He & Li Wang

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  1. Chendi Heng
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  2. Bowen He
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Contributions

Chendi Heng: Conceptualization, methodology, validation, analysis, investigation, data curation, writing—original draft preparation, and writing reviewing and editing. Bowen He: Data curation, validation, and investigation. Li Wang: Supervision, conceptualization, methodology, resources, validation, formal analysis, investigation, writing—original draft preparation, writing—reviewing and editing, project administration, and funding acquisition.

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Correspondence to Li Wang.

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Heng, C., He, B. & Wang, L. A Dual-mode Ratiometric Fluorometric and Colorimetric Platform Based on Nitrogen-doped Carbon Dots and o-phenylenediamine for the Detection of Nitrite. J Fluoresc (2023). https://doi.org/10.1007/s10895-023-03432-8

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