Abstract
In light of reverse chemical ecology, the fluorescence competitive binding assays of functional odorant binding proteins (OBPs) is a recent advanced approach for screening behaviorally active compounds of insects. Previous research on Dastareus helophoroides identified a minus-C OBP, DhelOBP21, which preferably binds to several ligands. In this study, only (+)-β-pinene proved attractive to unmated adult beetles. To obtain a more in-depth explanation of the lack of behavioral activity of other ligands we selected compounds with high (camphor) and low (β-caryophyllene) binding affinities. The structural transformation of OBPs was investigated using well-established approaches for studying binding processes, such as fluorescent quenching assays, circular dichroism, and molecular dynamics. The dynamic binding process revealed that the flexibility of DhelOBP21 seems conducive to binding specific ligands, as opposed to broad substrate binding. The compound (+)-β-pinene and DhelOBP21 formed a stable complex through a secondary structural transformation of DhelOBP21, in which its amino-terminus transformed from random coil to an α-helix to cover the binding pocket. On the other hand, camphor could not efficiently induce a stable structural transformation, and its high binding affinities were due to strong hydrogen-bonding, compromising the structure of the protein. The other compound, β-caryophyllene, only collided with DhelOBP21 and could not be positioned in the binding pocket. Studying structural transformation of these proteins through examining the dynamic binding process rather than using approaches that just measure binding affinities such as fluorescence competitive binding assays can provide a more efficient and reliable approach for screening behaviorally active compounds.
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Abbreviations
- DhelOBP21:
-
Dastarcus helophoroides odorant-binding protein 21
- GOBP:
-
General odorant binding protein
- PBP:
-
Pheromone binding protein
- CD:
-
Circular dichroism
- RNAi:
-
RNA interference
- qRT-PCR:
-
quantitative real-time PCR
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Acknowledgments
This study was supported and funded by the National Key Research and Development Program (2017YFD0600101), the National Natural Science Foundation of China (31230015) and Fundamental Research Funds for the Central Universities (2662015PY139).
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Yang, RN., Li, DZ., Yu, G. et al. Structural Transformation Detection Contributes to Screening of Behaviorally Active Compounds: Dynamic Binding Process Analysis of DhelOBP21 from Dastarcus helophoroides . J Chem Ecol 43, 1033–1045 (2017). https://doi.org/10.1007/s10886-017-0897-x
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DOI: https://doi.org/10.1007/s10886-017-0897-x