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Effect of Samarium Oxide Nanoparticles Fabricated by Curcumin on Efflux Pump and Virulence Genes Expression in MDR Pseudomonas aeruginosa and Staphylococcus aureus

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Abstract

The aim of this study was to prepare samarium oxide nanoparticles (Sm2O3NPs) using curcumin and investigation of antimicrobial activities of the prepared NPs. Sm2O3NPs were synthesized using curcumin and characterized by TEM, XRD, FTIR, FESEM, and EDX analyses. Antibacterial and antibiofilm activities of the synthesized NPs against Pseudomonas aeruginosa and Staphylococcus aureus were investigated using well diffusion and crystal violet staining methods, respectively. Finally, the expression of virulence-related genes (toxA, exoS, fnbA, and fnbB) and efflux pump genes (mexA, mexB, norA, and norB) in studied strains were determined using quantitative polymerase chain reaction. Characterization of the synthesized Sm2O3NPs showed that the NPs have a cubic structure with an average size of 32.61 nm, and a pure crystalline phase. The Sm2O3NPs displayed significant antibacterial activity against studied strains. Moreover, Sm2O3NPs efficiently inhibited biofilm formation of P. aeruginosa and S. aureus by 48 ± 3.29 and 53 ± 1%, respectively. Also, the synthesized Sm2O3NPs significantly reduced the expression of toxA, exoS, mexB, fnbA, norA, norB genes, by 44, 27, 31, 53, 40, and 30%, respectively. However, the expression of fnbB and mexA did not significantly change. This study confirmed the promising antibacterial potential of Sm2O3NPs, which could be employed as an effective antibacterial agent against clinically multidrug-resistant strains after further characterization.

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Authors and Affiliations

  1. Department of Microbiology, Lahijan Branch, Islamic Azad University, Lahijan, Iran

    Hossein Zahmatkesh, Mirsasan Mirpour & Behnam Rasti

  2. Department of Biology, University of Guilan, Rasht, Iran

    Hojjatolah Zamani

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  1. Hossein Zahmatkesh
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  2. Mirsasan Mirpour
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Correspondence to Mirsasan Mirpour.

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Zahmatkesh, H., Mirpour, M., Zamani, H. et al. Effect of Samarium Oxide Nanoparticles Fabricated by Curcumin on Efflux Pump and Virulence Genes Expression in MDR Pseudomonas aeruginosa and Staphylococcus aureus. J Clust Sci 34, 1227–1235 (2023). https://doi.org/10.1007/s10876-022-02274-x

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